-transparent.png){kind=logo}
Recombinant vaccinia viruses (VV) containing the envelope gene of bovine leukaemia virus (BLV) were constructed. Three virus constructs were designed: VV-BLV1 which contained the open reading frame for envelope glycoprotein gp51 alone, under control of VVP7.5 promoter; VV-BLV2 and VV-BLV3 contained the entire gene (gp51 + gp30) coding sequence downstream of VP7.5 and the fowlpox virus early/late promoter (PFE/L) respectively. All three VV recombinants expressed envelope glycoproteins as determined by the agar gel diffusion assay. By immunofluorescence techniques it was shown that while VV-BLV2 and VV-BLV3 expressed envelope glycoprotein on the surface of virus-infected cells, VV-BLV1 failed to do so. Rabbits inoculated with VV-BLV1 failed to show an anti envelope glycoprotein antibody response, however, significant levels of antibodies against envelope glycoprotein were detected in sera from rabbits inoculated with VV-BLV2 and VV-BLV3.
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
January 1986,
Nature,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
March 1991,
Vaccine,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
October 1988,
Vaccine,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
September 1989,
Journal of virology,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
January 1986,
Nature,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
November 1993,
Journal of virology,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
December 1989,
Acta virologica,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
November 2008,
Cytotechnology,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
September 1994,
Vaccine,
S Kumar, and
M E Andrew, and
D B Boyle, and
R B Brandon, and
M F Lavin, and
R C Daniel
August 1991,
Journal of virology,
