Biomaterial Database

A simple, sensitive assay for the spermicide nonoxynol-9 in biological fluids by high-performance liquid chromatography. 1990

G J Beck, and D Kossak, and S J Saxena

VLI Corporation, Irvine, CA 92714.

A new high-performance liquid chromatographic technique has been developed to quantitate nonoxynol-9 in serum, urine, and vaginal fluid. The method is rapid, involves minimal sample preparation, and can be used to analyze a large number of biological fluid samples. The assay elutes a single nonoxynol-9 peak with no interfering components. This was accomplished using a 10-microns pelicular packed amine column, a normal-phase solvent system, and fluorescence detection. Nonoxynol-9 levels as low as 0.23 micrograms/mL in urine can be detected.

UI	MeSH Term	Description	Entries
D011092	Polyethylene Glycols	Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.	Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D001826	Body Fluids	Liquid components of living organisms.	Body Fluid,Fluid, Body,Fluids, Body
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry
D013089	Spermatocidal Agents	Chemical substances that are destructive to spermatozoa used as topically administered vaginal contraceptives.	Spermicidal Agents,Contraceptive Agents, Spermatocidal,Spermatocidal Drugs,Spermatocides,Spermicidal Contraceptive Agents,Vaginal Spermicides,Agents, Spermatocidal,Agents, Spermatocidal Contraceptive,Agents, Spermicidal,Agents, Spermicidal Contraceptive,Contraceptive Agents, Spermicidal,Drugs, Spermatocidal,Spermatocidal Contraceptive Agents,Spermicides, Vaginal
D014621	Vagina	The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed)	Vaginas
D017137	Nonoxynol	Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Nonoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide, formulated primarily as a component of vaginal foams and creams.	Nonylphenoxypolyethoxyethanols,Advantage-S,Delfen Cream,Emulgen 911,Emulgin 913,Nonoxinol,Nonoxinols,Nonoxynol, 1(4)-Sulfate, Sodium Salt,Nonoxynol, 4-Sulfate, Ammonium Salt,Nonoxynol-9,Nonoxynols,Nonylphenoxypolyethoxyethanol,Patentex Oval,Advantage S,Delfen Creams,Nonoxynol 9,Oval, Patentex