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Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay. 2009
Qing Qu, and
Ziwen Zhu, and
Yufei Wang, and
Zhijun Zhong, and
Jin Zhao, and
Feng Qiao, and
Xinying Du, and
Zhoujia Wang, and
Ruifu Yang, and
Liuyu Huang, and
Yaqin Yu, and
Lei Zhou, and
Zeliang Chen
Department of Infectious Disease Control, Beijing Institute of Disease Control and Prevention, Beijing 100071, China.
A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6)CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.
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