BIOMAT Database Logo BIOMAT Database Logo

A combined physical and genetic map of Pseudomonas aeruginosa PAO. 1990

E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.

A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.

UI MeSH Term Description Entries
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003360 Cosmids Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles. Cosmid
D004251 DNA Transposable Elements Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. DNA Insertion Elements,DNA Transposons,IS Elements,Insertion Sequence Elements,Tn Elements,Transposable Elements,Elements, Insertion Sequence,Sequence Elements, Insertion,DNA Insertion Element,DNA Transposable Element,DNA Transposon,Element, DNA Insertion,Element, DNA Transposable,Element, IS,Element, Insertion Sequence,Element, Tn,Element, Transposable,Elements, DNA Insertion,Elements, DNA Transposable,Elements, IS,Elements, Tn,Elements, Transposable,IS Element,Insertion Element, DNA,Insertion Elements, DNA,Insertion Sequence Element,Sequence Element, Insertion,Tn Element,Transposable Element,Transposable Element, DNA,Transposable Elements, DNA,Transposon, DNA,Transposons, DNA
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D005816 Genetic Complementation Test A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell. Allelism Test,Cis Test,Cis-Trans Test,Complementation Test,Trans Test,Allelism Tests,Cis Tests,Cis Trans Test,Cis-Trans Tests,Complementation Test, Genetic,Complementation Tests,Complementation Tests, Genetic,Genetic Complementation Tests,Trans Tests
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot
D015183 Restriction Mapping Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction
D015252 Deoxyribonucleases, Type II Site-Specific Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4. DNA Restriction Enzymes, Type II,DNase, Site-Specific, Type II,Restriction Endonucleases, Type II,Type II Restriction Enzymes,DNase, Site Specific, Type II,Deoxyribonucleases, Type II, Site Specific,Deoxyribonucleases, Type II, Site-Specific,Site-Specific DNase, Type II,Type II Site Specific DNase,Type II Site Specific Deoxyribonucleases,Type II Site-Specific DNase,Type II Site-Specific Deoxyribonucleases,Deoxyribonucleases, Type II Site Specific,Site Specific DNase, Type II

Related Publications

E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
A physical genome map of Pseudomonas aeruginosa PAO.
December 1989, The EMBO journal,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
The chromosome map of Pseudomonas aeruginosa PAO.
January 1986, Acta microbiologica Polonica,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
A PacI/SwaI map of the Pseudomonas aeruginosa PAO chromosome.
January 1992, Electrophoresis,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Genetic circularity of the Pseudomonas aeruginosa PAO chromosome.
January 1981, Journal of bacteriology,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Genetic and physical mapping of genes involved in pyoverdin production in Pseudomonas aeruginosa PAO.
January 1995, Journal of bacteriology,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Chromosomal map of Pseudomonas putida PPN, and a comparison of gene order with the Pseudomonas aeruginosa PAO chromosomal map.
April 1985, Journal of general microbiology,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Genetic and physiological characterization of ciprofloxacin resistance in Pseudomonas aeruginosa PAO.
April 1988, Antimicrobial agents and chemotherapy,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Combined physical and genetic map of the Pseudomonas putida KT2440 chromosome.
December 1998, Journal of bacteriology,
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Genomic mapping of Pseudomonas aeruginosa PAO.
November 1994, Microbiology (Reading, England),
E Ratnaningsih, and S Dharmsthiti, and V Krishnapillai, and A Morgan, and M Sinclair, and B W Holloway
Isolation and genetic characterization of toxin-deficient mutants of Pseudomonas aeruginosa PAO.
August 1981, Journal of bacteriology,
Copied contents to your clipboard!