-transparent.png){kind=logo}
Fifty human cytomegalovirus (HCMV) isolates were recovered from different clinical specimens (buffy coat, throat washing and urine) obtained from fifty patients (23 AIDS patients, 20 heart transplant recipients, 1 bone marrow transplant recipient, 2 newborns with congenital HCMV infection and 4 immunocompetent individuals with acute HCMV infection). The isolates were previously identified by immunological methods and then examined for identification by the polymerase chain reaction. In parallel, reference HCMV strains as well as other human members of the Herpesviridae family (reference and wild strains) were examined as controls. Two pairs of primers relevant to the immediate-early and late regions of HCMV DNA, respectively, were used. The DNA amplification product was highly specific; in addition, all fifty HCMV isolates were amplified by both pairs of primers and thus identified as HCMV. These preliminary results show that selected pairs of primers are able to amplify DNA regions conserved enough to allow virus identification among a large number of HCMV strains. In addition, they are highly promising in view of the use of PCR for direct detection of HCMV DNA in clinical samples.
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
September 2002,
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
April 1995,
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
August 1989,
Journal of clinical microbiology,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
January 1995,
Rinsho byori. The Japanese journal of clinical pathology,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
December 1990,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
September 1993,
Journal of clinical microbiology,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
August 1993,
Australian and New Zealand journal of ophthalmology,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
January 2000,
Methods in molecular medicine,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
November 2006,
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica,
D Zipeto, and
E Silini, and
M Parea, and
E Percivalle, and
M Zavattoni, and
A Di Matteo, and
G Milanesi, and
G Gerna
November 1994,
Parasitology,
