Biomaterial Database

Characterization of subfractions from purified sarcolemma of canine left ventricle. 1990

D E Vatner

Department of Pediatrics, Harvard Medical School, Massachusetts General Hospital, Boston 02114.

Three membrane fractions were studied from canine myocardial left ventricle (LV); crude, light vesicle, and enriched sarcolemma. The percent of the total yield of membrane protein was 99.3 +/- 0.2% for the crude fraction, 0.4 +/- 0.1% for the light vesicle fraction, and 0.3 +/- 0.03% for the purified fraction. Sodium, potassium-ATPase activity in the purified fraction (100 +/- 10.8 mumol Pi/h/mg) was five fold more concentrated than in the light vesicle fraction (18.5 +/- 1.85 mumol Pi/h/mg), and nineteen fold more than in the crude fraction (5.29 +/- 0.57 mumol Pi/h/mg). beta-Adrenergic receptors were 8-fold enriched in the purified fraction (1006 +/- 219 vs 132 +/- 13 fmol/mg in the crude fraction) and 4-fold enriched in the light vesicle fraction (497 +/- 152 fmol/mg). Adenylate cyclase activity was enriched by only 13 to 17-fold in the purified fraction, and only 2 to 4-fold in the light vesicle fraction. The percent of the total beta-adrenergic receptors per gram wet weight was 94 +/- 20% for the crude fraction, 2.1 +/- 0.4% for the light vesicle fraction, and 3.8 +/- 0.7% for the purified fraction. When alamethicin was used to uncover latent enzyme activity, beta-adrenergic receptor density was not affected, but Na+,K(+)-ATPase and adenylate cyclase activity were enhanced in each membrane fraction studied. The surprising finding was that Na+,K(+)-ATPase activity was enriched to the same extent as the beta-adrenergic receptor density in the light vesicle fraction. One potential explanation is that the light vesicle fraction is located in a specialized region of the plasma membrane.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D011943	Receptors, Adrenergic, beta	One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.	Adrenergic beta-Receptor,Adrenergic beta-Receptors,Receptors, beta-Adrenergic,beta Adrenergic Receptor,beta-Adrenergic Receptor,beta-Adrenergic Receptors,Receptor, Adrenergic, beta,Adrenergic Receptor, beta,Adrenergic beta Receptor,Adrenergic beta Receptors,Receptor, beta Adrenergic,Receptor, beta-Adrenergic,Receptors, beta Adrenergic,beta Adrenergic Receptors,beta-Receptor, Adrenergic,beta-Receptors, Adrenergic
D002458	Cell Fractionation	Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.	Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D005260	Female		Females
D006352	Heart Ventricles	The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.	Cardiac Ventricle,Cardiac Ventricles,Heart Ventricle,Left Ventricle,Right Ventricle,Left Ventricles,Right Ventricles,Ventricle, Cardiac,Ventricle, Heart,Ventricle, Left,Ventricle, Right,Ventricles, Cardiac,Ventricles, Heart,Ventricles, Left,Ventricles, Right
D000254	Sodium-Potassium-Exchanging ATPase	An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.	ATPase, Sodium, Potassium,Adenosinetriphosphatase, Sodium, Potassium,Na(+)-K(+)-Exchanging ATPase,Na(+)-K(+)-Transporting ATPase,Potassium Pump,Sodium Pump,Sodium, Potassium ATPase,Sodium, Potassium Adenosinetriphosphatase,Sodium-Potassium Pump,Adenosine Triphosphatase, Sodium, Potassium,Na(+) K(+)-Transporting ATPase,Sodium, Potassium Adenosine Triphosphatase,ATPase Sodium, Potassium,ATPase, Sodium-Potassium-Exchanging,Adenosinetriphosphatase Sodium, Potassium,Pump, Potassium,Pump, Sodium,Pump, Sodium-Potassium,Sodium Potassium Exchanging ATPase,Sodium Potassium Pump
D000262	Adenylyl Cyclases	Enzymes of the lyase class that catalyze the formation of CYCLIC AMP and pyrophosphate from ATP.	Adenyl Cyclase,Adenylate Cyclase,3',5'-cyclic AMP Synthetase,Adenylyl Cyclase,3',5' cyclic AMP Synthetase,AMP Synthetase, 3',5'-cyclic,Cyclase, Adenyl,Cyclase, Adenylate,Cyclase, Adenylyl,Cyclases, Adenylyl,Synthetase, 3',5'-cyclic AMP
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012508	Sarcolemma	The excitable plasma membrane of a muscle cell. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)	Sarcolemmas