Total saponins of Panax ginseng induces K562 cell differentiation by promoting internalization of the erythropoietin receptor. 2009

Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China.

Ginseng is a commonly used herbal medicine with a wide range of therapeutic benefits. Total saponins of Panax ginseng (TSPG) is one of the main effective components of ginseng. Our previous studies have shown that TSPG could promote the production of normal blood cells and inhibition of the leukemia cell proliferation. However, whether ginseng can induce the differentiation of leukemia cells is still unclear. This study was to examine the effect of TSPG or the combination of erythropoietin (EPO) and TSPG on the erythroid differentiation of K562 cells, and their corresponding mechanisms regarding erythropoietin receptor (EPOR) expression. Under light and electron microscopes, the TSPG- or TSPG + EPO-treated K562 cells showed a tendency to undergo erythroid differentiation; early and intermediate erythroblast-like cells were observed. Hemoglobin and HIR2 expressions were significantly increased. As determined by Western blotting analysis, the EPOR protein level in the K562 cytoplasmic membrane was significantly decreased after TSPG treatment, while its cytoplasm level increased in a dose-dependent manner. However, the total cellular EPOR level was unchanged. These results indicate that TSPG-induced erythroid differentiation of K562 cells may be accompanied by the internalization of EPOR. Thus, our study suggests that treatment with a combination of TSPG and EPO may induce erythroid differentiation of K562 cells at least in part through induction of EPOR internalization.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004705 Endocytosis Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis. Endocytoses
D004900 Erythroblasts Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts. Erythrocytes, Nucleated,Normoblasts,Proerythroblasts,Pronormoblasts,Erythroblast,Erythrocyte, Nucleated,Normoblast,Nucleated Erythrocyte,Nucleated Erythrocytes,Proerythroblast,Pronormoblast
D004921 Erythropoietin Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005894 Panax An araliaceous genus of plants that contains a number of pharmacologically active agents used as stimulants, sedatives, and tonics, especially in traditional medicine. Sometimes confused with Siberian ginseng (ELEUTHEROCOCCUS). Ginseng,Korean Ginseng,Korean Red Ginseng,Panax ginseng,Jen Shen,Ninjin,Renshen,Schinseng,Shinseng,Ginseng, Korean,Ginseng, Korean Red,Ginsengs,Jen Shens,Korean Ginsengs,Korean Red Ginsengs,Ninjins,Red Ginseng, Korean,Renshens,Schinsengs,Shen, Jen,Shinsengs
D006454 Hemoglobins The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements. Eryhem,Ferrous Hemoglobin,Hemoglobin,Hemoglobin, Ferrous

Related Publications

Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
November 1995, Phytochemistry,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
October 2015, Journal of ginseng research,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
November 2011, Journal of ethnopharmacology,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
October 1988, Chemical & pharmaceutical bulletin,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
April 2023, Journal of pharmaceutical analysis,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
April 2007, Nan fang yi ke da xue xue bao = Journal of Southern Medical University,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
January 2000, Pharmaceutical biology,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
January 1999, Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine,
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
August 2003, Sheng li xue bao : [Acta physiologica Sinica],
Guowei Zuo, and Tao Guan, and Dilong Chen, and Chunli Li, and Rong Jiang, and Chunyan Luo, and Xiaoshu Hu, and Yaping Wang, and Jianwei Wang
January 2015, Chemical & pharmaceutical bulletin,
Copied contents to your clipboard!