BIOMAT Database Logo BIOMAT Database Logo

Modulation of muscarinic signaling in PC12 cells overexpressing neuronal Ca2+ sensor-1 protein. 2009

M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Faculdade de Medicina da UFMGAv Alfredo Balena Laboratorio de Neurociência, Departamento de Saude Mental, INCT de Medicina Molecular Brazil.

It has been suggested that overexpression of neuronal Ca2+ sensor-1 (NCS-1) protein is implicated in the pathophysiology of neurodisorders such as schizophrenia, bipolar disturbance and X-linked mental retardation. The mechanism by which NCS-1 would be involved in the causes and/or consequences of these neurodisorders is still far from elucidation. Independent evidence has pointed NCS-1 as a key regulator of synaptic efficacy by altering the expression and activity of voltage-gated channels, inhibiting internalization of dopaminergic receptors, and altering phosphoinositide metabolism. In this study, we examined the possible participation of NCS-1 protein in signal transmission dependent on muscarinic receptor activation, using PC12 cells stably expressing NCS-1 (PC12-NCS-1). Carbachol (CCH; 300 microM) was able to evoke glutamate release more efficiently from PC12-NCS-1 (15.3+/-1.0nmol/mg of protein) than wild type cells (PC12-wt; 8.3+/-0.9nmol/mg of protein). This increase of glutamate release induced by CCH was independent on extracellular Ca2+ influx. Additionally, a larger increase of cytoplasmic levels of InsP3 (663.0+/-63.0 and 310.0+/-39.0% of fluorescence in A.U.) and [Ca2+]i (766.4+/-40.0 and 687.8+/-37.1nmol/L) was observed after CCH stimulus of PC12-NCS-1 compared with PC12-wt. Clearly distinction between intracellular Ca2+ dynamics was also observed in PC12-NCS-1 and PC12-wt. A larger increase followed by fast decay of [Ca2+]i was observed in PC12-NCS-1. A plateau with a delayed decay of [Ca2+]i was characteristic of PC12-wt [Ca2+]i response. Both enhancement of InsP3 production and glutamate release observed in PC12-NCS-1 were blocked by atropine (10 microM). Together, our data show that overexpression of NCS-1 in PC12 cells induces an enhancement of intracellular second messenger and transmitter release dependent on CCH response, suggesting that muscarinic signaling is "up-regulated" in this cell model.

UI MeSH Term Description Entries
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D009479 Neuropeptides Peptides released by NEURONS as intercellular messengers. Many neuropeptides are also hormones released by non-neuronal cells. Neuropeptide
D011976 Receptors, Muscarinic One of the two major classes of cholinergic receptors. Muscarinic receptors were originally defined by their preference for MUSCARINE over NICOTINE. There are several subtypes (usually M1, M2, M3....) that are characterized by their cellular actions, pharmacology, and molecular biology. Muscarinic Acetylcholine Receptors,Muscarinic Receptors,Muscarinic Acetylcholine Receptor,Muscarinic Receptor,Acetylcholine Receptor, Muscarinic,Acetylcholine Receptors, Muscarinic,Receptor, Muscarinic,Receptor, Muscarinic Acetylcholine,Receptors, Muscarinic Acetylcholine
D011993 Recombinant Fusion Proteins Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. Fusion Proteins, Recombinant,Recombinant Chimeric Protein,Recombinant Fusion Protein,Recombinant Hybrid Protein,Chimeric Proteins, Recombinant,Hybrid Proteins, Recombinant,Recombinant Chimeric Proteins,Recombinant Hybrid Proteins,Chimeric Protein, Recombinant,Fusion Protein, Recombinant,Hybrid Protein, Recombinant,Protein, Recombinant Chimeric,Protein, Recombinant Fusion,Protein, Recombinant Hybrid,Proteins, Recombinant Chimeric,Proteins, Recombinant Fusion,Proteins, Recombinant Hybrid
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002217 Carbachol A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS. Carbamylcholine,Carbacholine,Carbamann,Carbamoylcholine,Carbastat,Carbocholine,Carboptic,Doryl,Isopto Carbachol,Jestryl,Miostat,Carbachol, Isopto
D002614 Chelating Agents Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS. Chelating Agent,Chelator,Complexons,Metal Antagonists,Chelators,Metal Chelating Agents,Agent, Chelating,Agents, Chelating,Agents, Metal Chelating,Antagonists, Metal,Chelating Agents, Metal
D004533 Egtazic Acid A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID. EGTA,Ethylene Glycol Tetraacetic Acid,EGATA,Egtazic Acid Disodium Salt,Egtazic Acid Potassium Salt,Egtazic Acid Sodium Salt,Ethylene Glycol Bis(2-aminoethyl ether)tetraacetic Acid,Ethylenebis(oxyethylenenitrile)tetraacetic Acid,GEDTA,Glycoletherdiamine-N,N,N',N'-tetraacetic Acid,Magnesium-EGTA,Tetrasodium EGTA,Acid, Egtazic,EGTA, Tetrasodium,Magnesium EGTA
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections

Related Publications

M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Modulation of a Ca2+ signaling pathway by GM1 ganglioside in PC12 cells.
December 1992, The Journal of biological chemistry,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Zonisamide attenuates MPP+-induced oxidative toxicity through modulation of Ca2+ signaling and caspase-3 activity in neuronal PC12 cells.
March 2013, Cellular and molecular neurobiology,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Mechanisms underlying the neuronal calcium sensor-1-evoked enhancement of exocytosis in PC12 cells.
August 2002, The Journal of biological chemistry,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
PC12 cells overexpressing the insulin receptor undergo insulin-dependent neuronal differentiation.
August 1994, Current biology : CB,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Activation of muscarinic receptors in PC12 cells. Stimulation of Ca2+ influx and redistribution.
March 1986, The Biochemical journal,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Alterations in exocytosis induced by neuronal Ca2+ sensor-1 in bovine chromaffin cells.
April 2002, The Journal of neuroscience : the official journal of the Society for Neuroscience,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Implication of neuronal Ca2+ -sensor protein VILIP-1 in the glutamate hypothesis of schizophrenia.
October 2008, Neurobiology of disease,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Neuronal Ca2+ sensor 1, the mammalian homologue of frequenin, is expressed in chromaffin and PC12 cells and regulates neurosecretion from dense-core granules.
August 1998, The Journal of biological chemistry,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Muscarinic activation of mitogen-activated protein kinase in PC12 cells.
August 2000, Journal of neurochemistry,
M M Guimarães, and H J Reis, and L P Guimarães, and D S Carneiro, and F M Ribeiro, and M V Gomez, and A Jeromin, and M A Romano-Silva
Modifications of Ca2+ signaling by inorganic mercury in PC12 cells.
December 1993, FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
Copied contents to your clipboard!