-transparent.png){kind=logo}
Photodynamic therapy is a new concept for in vitro evaluation of bone marrow clearance in leukemia. This treatment eliminates 99.9999% of leukemic cells, but under the same conditions over 50% of normal bone marrow cells are damaged. WR-2721, a thiol compound, is reported to except a protective effect for bone marrow against radiation therapy. This study analyzed the protective effect of WR-2721 during photodynamic therapy with hematoporphyrin derivative. Mice hemopoietic cells were exposed to laser light after sensitization by hematoporphyrin, with or without WR-2721 at 3 dose levels (250, 500, 750 mg/kg). The efficacy of protection was evaluated by GM-CFU assay in collagen gel medium. Results showed significant protection at 25 and 50 j/cm2 (p less than 0.05), but at 75 j/cm2 irradiation only the 750 mg/kg dosage remained protective. The protective factor of WR-2721 is 1.65 (95% confidence interval: 1.38-1.99) WR-2721 is protective against photodynamic lesions but this protection is diminished by increased irradiation energy. The best results are obtained with a dose of 750 mg/kg though this appears to be toxic. Further studies are needed to evaluate the action of WR-2721 on leukemic cells before the use of WR-2721 in bone marrow clearance.
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
March 1987,
Radiation research,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
February 1984,
Radiation research,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
August 1988,
Photochemistry and photobiology,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
November 1984,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
November 1982,
Cancer research,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
October 1989,
International journal of radiation oncology, biology, physics,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
June 1988,
Cancer,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
January 1992,
Kidney international,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
April 1980,
Radiation research,
F Maloisel, and
M T Foultier, and
T Patrice, and
V Praloran, and
F Oberling, and
L Le Bodic
September 2000,
Leukemia,
