BIOMAT Database Logo BIOMAT Database Logo

cDNA-cloning and expression of VP1-specific sequences of foot-and-mouth disease virus types A5 and O1. 1990

L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
Friedrich-Loeffler-Institut Insel Riems.

The RNA genome of foot- and mouth disease virus strains A5 Westerwald and O1 Lausanne has been reverse-transcribed and cloned in lambdaphages or plasmids. Identification of cDNA-clones containing VP1-specific sequences was achieved by hybridization, restriction mapping, and sequence analysis. VP1-coding cDNA-fragments were subcloned into the expression vector pEX which led to synthesis of fusion proteins with beta-galactosidase. These fusion proteins reacted with anti-VP1 antibodies on a Western blot, but were not capable of inducing neutralizing antibodies to mice. This seemed to suggest a tertiary structure of the VP1-epitopes unlike those of native VP1. Other attempts are discussed to construct VP1-fusion proteins folding similarly to the native viral protein structure.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005537 Aphthovirus A genus of the family PICORNAVIRIDAE infecting mainly cloven-hoofed animals. They cause vesicular lesions and upper respiratory tract infections. FOOT AND MOUTH DISEASE VIRUS is the type species. Equine rhinitis A virus,Equine rhinovirus 1,Aphthoviruses
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012689 Sequence Homology, Nucleic Acid The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function. Base Sequence Homology,Homologous Sequences, Nucleic Acid,Homologs, Nucleic Acid Sequence,Homology, Base Sequence,Homology, Nucleic Acid Sequence,Nucleic Acid Sequence Homologs,Nucleic Acid Sequence Homology,Sequence Homology, Base,Base Sequence Homologies,Homologies, Base Sequence,Sequence Homologies, Base
D014764 Viral Proteins Proteins found in any species of virus. Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D015967 Gene Expression Regulation, Viral Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses. Regulation of Gene Expression, Viral,Viral Gene Expression Regulation,Regulation, Gene Expression, Viral

Related Publications

L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
VP1-coding sequences of recent isolates of foot-and-mouth disease virus types A, O and Asia1.
January 1998, Virus genes,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
Immunoglobulin VH and VK genes of the BALB/c anti-foot-and-mouth disease virus (O1) VP1 response: cloning, characterization and transgenic mice.
November 1991, Molecular immunology,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
Molecular cloning of cDNA from foot-and-mouth disease virus C1-Santa Pau (C-S8). Sequence of protein-VP1-coding segment.
August 1983, Gene,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli.
February 1981, Nature,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
Radioimmunoassay for detection of VP1 specific neutralizing antibodies of foot and mouth disease virus.
January 1985, Journal of virological methods,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
New virus-specific T-helper epitopes of foot-and-mouth disease viral VP1 protein.
October 1993, FEBS letters,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
New virus-specific T-helper epitopes of foot-and-mouth disease viral VP1 protein.
January 1994, FEBS letters,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
Production of foot-and-mouth disease virus SAT2 VP1 protein.
January 2020, AMB Express,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
'Hand, foot, and mouth disease' associated with Coxsackie A5 virus.
January 1963, Journal of clinical pathology,
L Meyer, and H W Heinrich, and M Lenk, and S Kleinwächter, and H Horstmann, and S Bochnig, and P Eichler, and P Kruschke
Foot-and-mouth disease virus subtyping by sequencing VP1 genes.
June 1990, Veterinary microbiology,
Copied contents to your clipboard!