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Rabbit articular chondrocytes in suspension culture synthesize Type II collagen [3alpha1(II)] in the absence of extracellular Ca2+ and Type I collagen [2alpha1(I) - alpha2] in the complete medium. As a result of pre-treatment in monolayer culture with calcitonin or parathyroid hormone in the complete medium, an influx of Ca2+ into the cells occurs. These cells produce mainly Type I collagen when transferred to suspension cultures in the medium devoid of CaCl2. If added directly to the suspension culture medium containing no CaCl2, calcitonin stimulates an active efflux of Ca2+ from the cells into the medium and leads the cells to synthesize Type I collagen. Under similar conditions, parathyroid hormone does not change the collagen-phenotype.
K Deshmukh, and
W G Kline, and
B D Sawyer
July 1981,
General and comparative endocrinology,
K Deshmukh, and
W G Kline, and
B D Sawyer
March 1997,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
K Deshmukh, and
W G Kline, and
B D Sawyer
July 1979,
Calcified tissue international,
K Deshmukh, and
W G Kline, and
B D Sawyer
February 1975,
Endocrinology,
K Deshmukh, and
W G Kline, and
B D Sawyer
February 1974,
The Journal of cell biology,
K Deshmukh, and
W G Kline, and
B D Sawyer
September 1983,
Nihon Kyosei Shika Gakkai zasshi = The journal of Japan Orthodontic Society,
K Deshmukh, and
W G Kline, and
B D Sawyer
April 1980,
Kidney international,
K Deshmukh, and
W G Kline, and
B D Sawyer
October 1980,
Endocrinology,
K Deshmukh, and
W G Kline, and
B D Sawyer
September 1969,
Casopis lekaru ceskych,
K Deshmukh, and
W G Kline, and
B D Sawyer
January 1969,
Annual review of pharmacology,
