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A cDNA encoding potato (Solanum tuberosum L.) 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway, was cloned into phage lambda gt11. The clone represents the first cDNA for this enzyme from any eukaryotic source. The nucleotide sequence of the cDNA was determined, and its identity was confirmed through partial amino acid sequence analysis of the encoded enzyme. The cDNA contains a 1527-base pair open reading frame that encodes a polypeptide with a calculated molecular weight of 56,153. The amino terminus of the deduced polypeptide resembles a chloroplast transit sequence. Amino acid sequence identities between the mature potato enzyme and the homologous isoenzymes from Escherichia coli are only about 22%. The potato cDNA hybridized to various plant mRNAs that are all about 2 kilobases in size.
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
December 1986,
Plant physiology,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
October 1992,
Plant physiology,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
February 1992,
Plant physiology,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
August 1988,
Plant physiology,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
August 2002,
Plant physiology,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
May 1979,
The Journal of biological chemistry,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
November 1990,
Journal of bacteriology,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
October 1991,
Plant physiology,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
July 2005,
Proceedings of the National Academy of Sciences of the United States of America,
W E Dyer, and
L M Weaver, and
J M Zhao, and
D N Kuhn, and
S C Weller, and
K M Herrmann
February 1993,
Biochimica et biophysica acta,
