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A mAb, 10D1, was obtained by fusing spleen cells from BALB/c mice immunized with a CD3/TCR- human T cell line, P12/ichikawa, to mouse myeloma cells, P3X63-Ag8-653. 10D1 mAb is specific for T cells in that it reacted with all the T cell lines tested, but not with B or myeloid cell lines. A small fraction of normal peripheral blood T cells, preferentially CD4+, was also reactive with 10D1 mAb. Biochemical studies revealed that 10D1 mAb recognizes a disulfide-linked homodimeric molecule composed of 90-kDa polypeptide. 10D1 mAb induced a substantial proliferation of peripheral blood T cells when cross-linked with goat anti-mouse Ig antibody. The elimination of CD4+ cells totally abrogated the proliferative response induced by 10D1 mAb, whereas the elimination of CD8+ cells rather enhanced it. The proliferative response of peripheral blood T cells induced by 10D1 mAb was almost completely inhibited after modulation of the CD3/TCR complex with anti-CD3 mAb. In addition, a prompt increase in intracellular [Ca2+] was observed in a CD3+ T cell line, Jurkat but not in its surface CD3- mutant when 10D1 mAb was added. These results indicate that the 10D1 molecule is involved in a novel pathway of human CD4+ T cell activation, which is associated with the CD3/TCR-mediated pathway.
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
November 1989,
European journal of immunology,
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
October 1987,
European journal of immunology,
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
July 1995,
Nature,
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
December 1989,
Immunology today,
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
December 1993,
Cellular immunology,
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
September 1991,
Journal of immunology (Baltimore, Md. : 1950),
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
March 1990,
Cellular immunology,
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
January 1986,
Current topics in microbiology and immunology,
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
September 2003,
Journal of immunology (Baltimore, Md. : 1950),
T Kobata, and
H Yagita, and
H Matsuda, and
S Tansyo, and
H Yakura, and
M Katagiri, and
K Okumura
September 1991,
European journal of immunology,
