BIOMAT Database Logo BIOMAT Database Logo

A method for typing strains of Legionella pneumophila serogroup 1 by analysis of restriction fragment length polymorphisms. 1990

N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Legionella Reference Unit, DMRQC, Central Public Health Laboratory, London.

A restriction fragment length polymorphism (RFLP) typing method for Legionella pneumophila serogroup 1 was developed. The method depended upon the use of cloned EcoR1 fragments from L. pneumophila (Knoxville-1) probing Nci1 restriction fragments of chromosomal DNA. Examination of strains of L. pneumophila which were apparently unrelated showed that inter-strain RFLPs were common, and these formed the basis of the typing scheme. The technique was found to be highly reproducible and discriminatory. When the RFLP data were compared to that obtained by monoclonal antibody (MAb) subgrouping both methods of strain differentiation gave consistent results. The isolates examined by either method were also sub-divided by the alternative technique. The analysis of RFLPs by cloned probes should be of considerable epidemiological value.

UI MeSH Term Description Entries
D007875 Legionella Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot
D015342 DNA Probes Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. Chromosomal Probes,DNA Hybridization Probe,DNA Probe,Gene Probes, DNA,Conserved Gene Probes,DNA Hybridization Probes,Whole Chromosomal Probes,Whole Genomic DNA Probes,Chromosomal Probes, Whole,DNA Gene Probes,Gene Probes, Conserved,Hybridization Probe, DNA,Hybridization Probes, DNA,Probe, DNA,Probe, DNA Hybridization,Probes, Chromosomal,Probes, Conserved Gene,Probes, DNA,Probes, DNA Gene,Probes, DNA Hybridization,Probes, Whole Chromosomal

Related Publications

N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Subtyping of Legionella pneumophila serogroup 1 isolates by small-fragment restriction endonuclease analysis.
August 1991, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Molecular typing of Legionella pneumophila serogroup 1 by pulsed-field gel electrophoresis with SfiI and comparison of this method with restriction fragment-length polymorphism analysis.
March 1999, Journal of medical microbiology,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Molecular typing of Legionella pneumophila serogroup 1 clinical strains isolated in Italy.
July 2014, International journal of medical microbiology : IJMM,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Assessment of fluorescent amplified fragment length polymorphism analysis for epidemiological genotyping of Legionella pneumophila serogroup 1.
September 2005, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Comparative analysis of infrequent-restriction-site PCR and pulsed-field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains.
January 1998, Journal of clinical microbiology,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
DNA typing: analysis of restriction fragment length polymorphisms.
January 1994, Australasian biotechnology,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
An epidemiologically valuable typing method for Neisseria meningitidis by analysis of restriction fragment length polymorphisms.
May 1991, Journal of medical microbiology,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
[Legionella pneumophila serogroup 1].
October 2003, Enfermedades infecciosas y microbiologia clinica,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Assessment of intercentre reproducibility and epidemiological concordance of Legionella pneumophila serogroup 1 genotyping by amplified fragment length polymorphism analysis.
October 2000, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
N A Saunders, and T G Harrison, and A Haththotuwa, and N Kachwalla, and A G Taylor
Typing of Legionella pneumophila serogroup 1 isolates by degenerate (D-)RAPD fingerprinting.
December 1995, Molecular and cellular probes,
Copied contents to your clipboard!