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The presence of a new lactate dehydrogenase inhibitor on the trailing edge of the NADH peak from chromatography on diethylaminoethyl-celluose [Loshon et al., Clin. Chem., this issue] was verified. It was resolved from the NADH by high-performance liquid chromatography on muBondapak C18. When the new inhibitor was present in a reaction mixture to the extent that, of the initial 260-nm absorbance, about 5% was contributed by the inhibitor, the rate of NADH oxidation by lactate dehydrogenase decreased by 65%. The inhibitor absorbs at 260 and 340 nm, and is different from the Strandjord-Clayson inhibitor [J. Lab. Clin. Med. 67, 144 (1966)] by both types of chromatography. Because this new inhibitor has ultraviolet properties similar to those of NADH and chromatographs with the NADH on DEAE-cellulose, the high-performance liquid chromatographic method must be used to ensure its absence in preparations of NADH used for clinical assay.
S A Margolis, and
B F Howell, and
R Schaffer
September 1977,
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S A Margolis, and
B F Howell, and
R Schaffer
November 1973,
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S A Margolis, and
B F Howell, and
R Schaffer
April 1978,
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S A Margolis, and
B F Howell, and
R Schaffer
January 1977,
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S A Margolis, and
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S A Margolis, and
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S A Margolis, and
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S A Margolis, and
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S A Margolis, and
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S A Margolis, and
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R Schaffer
September 1989,
Proceedings of the National Academy of Sciences of the United States of America,
