Validation of the procedure for the determination of maduramicin in concentrates, premixes, and feeds by liquid chromatography. 2009

G Tavcar-Kalcher, and K Pavsic-Vrtac, and A Vengust
Veterinary Faculty-National Veterinary Institute, University in Ljubljana, Ljubljana, Slovenia. gabrijela.tavcar@vf.uni-lj.si

A single laboratory validation was carried out for the determination of maduramicin in concentrates, premixes, and feed. The method comprised sample extraction of maduramicin, derivatization with dansylhydrazine and liquid chromatography with ultraviolet light detection. The limit of detection (LOD) and limit of quantification (LOQ) were 0.4 and 1.0 mg kg(-1), respectively. The repeatability expressed as the average difference between the results of duplicate measurements was 5.9% at the concentration level of 1% (concentrate), 7.1% at the concentration level of 1 g kg(-1) (premix), and 11% with the feed containing maduramicin with the nominal concentration of 5 mg kg(-1) and feed spiked at the concentration level of 1 mg kg(-1). The relative standard deviations for the within-laboratory reproducibility (RSD(W)) were 9.2%, 16%, 18%, and 17% at the concentration levels of 1%, 1 g kg(-1), 5 mg kg(-1), and 1 mg kg(-1), respectively. The measurement uncertainties were +/-0.2%, +/-0.3 g kg(-1), +/-1.9 mg kg(-1), and +/-0.3 mg kg(-1) at the same concentration levels, respectively.

UI MeSH Term Description Entries
D007783 Lactones Cyclic esters of hydroxy carboxylic acids, containing a 1-oxacycloalkan-2-one structure. Large cyclic lactones of over a dozen atoms are MACROLIDES. Lactone
D011200 Poultry Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others. Fowls, Domestic,Domestic Fowl,Domestic Fowls,Fowl, Domestic,Poultries
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D003049 Coccidiostats Agents useful in the treatment or prevention of COCCIDIOSIS in man or animals. Anticoccidial Agents,Agents, Anticoccidial
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000821 Animal Feed Foodstuff used especially for domestic and laboratory animals, or livestock. Fodder,Animal Feeds,Feed, Animal,Feeds, Animal,Fodders
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D057230 Limit of Detection Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure. Limits of Detection,Detection Limit,Detection Limits

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