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The effects of pertussis toxin (PTX) on synaptosomal tyrosine hydroxylase (TH) activity and on the inhibition of synaptosomal TH activity by apomorphine were investigated. Exposure of striatal synaptosomes to PTX does not affect basal- or forskolin-stimulated TH activity, but attenuates apomorphine-elicited inhibition of forskolin-stimulated synaptosomal TH activity. There is a good correlation between the attenuation of apomorphine-elicited inhibition of synaptosomal TH activity by PTX and (-)-sulpiride, suggesting that G proteins are involved in the dopamine (DA) autoreceptor-mediated regulation of the enzyme activity. The exposure of synaptosome to PTX results in a 40-50% inactivation of Gi and Go proteins, which is evident from the reduction of ADP ribosylation with [32P]NAD of the remaining G proteins following preincubation with the toxin. The present study also demonstrates that striatal synaptosomal preparations can be used for investigations of the molecular properties of nerve terminal DA autoreceptors.
K Harada, and
E Meller, and
M Goldstein
January 1975,
Psychopharmacology communications,
K Harada, and
E Meller, and
M Goldstein
September 1974,
The Journal of pharmacy and pharmacology,
K Harada, and
E Meller, and
M Goldstein
May 1974,
The Journal of pharmacy and pharmacology,
K Harada, and
E Meller, and
M Goldstein
January 1975,
Psychopharmacology communications,
K Harada, and
E Meller, and
M Goldstein
September 1984,
Naunyn-Schmiedeberg's archives of pharmacology,
K Harada, and
E Meller, and
M Goldstein
December 1974,
The Journal of pharmacy and pharmacology,
K Harada, and
E Meller, and
M Goldstein
October 1980,
Psychopharmacology bulletin,
K Harada, and
E Meller, and
M Goldstein
September 1990,
Brain research,
K Harada, and
E Meller, and
M Goldstein
December 1975,
Life sciences,
K Harada, and
E Meller, and
M Goldstein
January 1991,
Neurotoxicology and teratology,
