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The human NUDT5 protein catalyzes the hydrolysis of 8-hydroxy-dGDP. To examine its substrate specificity, four oxidized deoxyribonucleotides (2-hydroxy-dADP, 8-hydroxy-dADP, 5-formyl-dUDP, and 5-hydroxy-dCDP) were incubated with the NUDT5 protein. Interestingly, all of the nucleotides, except for 5-hydroxy-dCDP, were hydrolyzed with various efficiencies. The kinetic parameters indicated that 8-hydroxy-dADP was hydrolyzed as efficiently as 8-hydroxy-dGDP. The hydrolyzing activities for their triphosphate counterparts were quite weak. These results suggest that the NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects.
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
November 2011,
Fukuoka igaku zasshi = Hukuoka acta medica,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
February 1985,
Journal of biomolecular structure & dynamics,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
June 2012,
The Journal of biological chemistry,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
September 2020,
Insect biochemistry and molecular biology,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
February 2007,
The Journal of biological chemistry,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
October 1961,
The Journal of biological chemistry,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
April 1975,
Biochemical and biophysical research communications,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
February 2004,
FEBS letters,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
December 2006,
Journal of molecular biology,
Hiroyuki Kamiya, and
Mika Hori, and
Takao Arimori, and
Mutsuo Sekiguchi, and
Yuriko Yamagata, and
Hideyoshi Harashima
March 2003,
Nucleic acids research,
