OBJECTIVE Preparation of reusable and easy to handle Celite chromatographic columns. METHODS Weighting precise Celite quantities in cartridges and introducing ethylene glycol methanol solutions. The chromatographic solvents pass throughout Celite under negative pressure. RESULTS These new minicolumns are reusable. The steroid recoveries' coefficients of variation are less than 10%, and the steroid separation is good. CONCLUSIONS The reusable Celite cartridge use before steroid immunoassays is easier and less time-consuming than classical glass Celite minicolumns.