Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry. 2009

Souad Hadjoudja, and Chantal Vignoles, and Véronique Deluchat, and Jean-François Lenain, and Anne-Hélène Le Jeune, and Michel Baudu
Université de Limoges, GRESE EA 4330, 123 avenue Albert Thomas 87060, Limoges, France.

Copper sulphate is a common algaecide applied to ponds in order to keep phytoplanktonic blooms under control, especially those prone to cyanobacterial development. The copper toxicity was evaluated for two phytoplanktonic species with the same size and shape: one cyanobacteria Microcystis aeruginosa and one chlorophyceae Chlorella vulgaris. Under controlled conditions in order to keep free copper concentration constant, three bioassays were carried out: growth inhibition tests and two sets of flow cytometric analysis (autofluorescence and esterase activity inhibition). The results showed: (1) that regardless of the cell type, as copper concentrations increased, cell division rate and autofluorescence decreased, (2) a difference in sensitivity according to the length of exposure to copper: 24 or 48 h, and (3) that M. aeruginosa showed a greater sensitivity than C. vulgaris in the 24h copper exposure assay according to esterase activity data.

UI MeSH Term Description Entries
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D003300 Copper A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55. Copper-63,Copper 63
D004950 Esterases Any member of the class of enzymes that catalyze the cleavage of an ester bond and result in the addition of water to the resulting molecules. Esterase
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D001681 Biological Assay A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc. Bioassay,Assay, Biological,Assays, Biological,Biologic Assay,Biologic Assays,Assay, Biologic,Assays, Biologic,Bioassays,Biological Assays
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D046931 Microcystis A form-genus of CYANOBACTERIA in the order Chroococcales. Many species are planktonic and possess gas vacuoles. Microcystis aeruginosa
D048408 Chlorella vulgaris A species of green microalgae in the family Chlorellaceae. It is used as a model organism for PHOTOSYNTHESIS, and as a food supplement (DIETARY SUPPLEMENTS). Chlorella vulgari,vulgaris, Chlorella
D018675 Toxicity Tests An array of tests used to determine the toxicity of a substance to living systems. These include tests on clinical drugs, foods, and environmental pollutants. Tests, Toxicity,Test, Toxicity,Toxicity Test
D019327 Copper Sulfate A sulfate salt of copper. It is a potent emetic and is used as an antidote for poisoning by phosphorus. It also can be used to prevent the growth of algae. Cupric Sulfate,Blue Vitriol,Sulfate, Copper,Sulfate, Cupric,Vitriol, Blue

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