Ghrelin potentiates miniature excitatory postsynaptic currents in supraoptic magnocellular neurones. 2009

T Yokoyama, and T Saito, and T Ohbuchi, and H Suzuki, and H Otsubo, and T Okamoto, and H Fujihara, and T Nagatomo, and Y Ueta
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.

Ghrelin is an orexigenic peptide discovered in the stomach as a ligand of the orphan G-protein coupled receptor, and participates in the regulation of growth hormone (GH) release. Previous studies have demonstrated that ghrelin suppressed water intake and stimulated the secretion of arginine vasopressin in rats. We examined the effect of ghrelin on the excitatory synaptic inputs to the magnocellular neurosecretory cells (MNCs) in the supraoptic nucleus (SON) using whole-cell patch-clamp recordings in in vitro rat and mouse brain slice preparations. The application of ghrelin (10(-7) approximately 10(-6) m) caused a significant increase in the frequency of the miniature excitatory postsynaptic currents (mEPSCs) in a dose-related manner without affecting the amplitude. The increased frequency of the spontaneous EPSCs persisted in the presence of tetrodotoxin (1 microM). Des-n-octanoyl ghrelin (10(-6) m) did not have a significant effect on the mEPSCs. The ghrelin-induced potentiation of the mEPSCs was significantly suppressed by previous exposure to the transient receptor potential vanilloid (TRPV) blocker, ruthenium red (10 microM) and GH secretagougue type 1a receptor selective antagonist, BIM28163 (10 microM). The effects of ghrelin on the supraoptic MNCs in trpv1 knockout mice were significantly attenuated compared to those in wild-type mice counterparts. These results suggest that ghrelin participates in the regulation of synaptic inputs to the MNCs in the SON via interaction with the GH secretagogue type 1a receptor, and that the TRPV1 channel may be involved in ghrelin-induced potentiation of mEPSCs to the MNCs in the SON.

UI MeSH Term Description Entries
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013495 Supraoptic Nucleus Hypothalamic nucleus overlying the beginning of the OPTIC TRACT. Accessory Supraoptic Group,Nucleus Supraopticus,Supraoptic Nucleus of Hypothalamus,Accessory Supraoptic Groups,Group, Accessory Supraoptic,Groups, Accessory Supraoptic,Hypothalamus Supraoptic Nucleus,Nucleus, Supraoptic,Supraoptic Group, Accessory,Supraoptic Groups, Accessory,Supraopticus, Nucleus
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D054439 Ghrelin A 28-amino acid, acylated, orexigenic peptide that is a ligand for GROWTH HORMONE SECRETAGOGUE RECEPTORS. Ghrelin is widely expressed but primarily in the stomach in the adults. Ghrelin acts centrally to stimulate growth hormone secretion and food intake, and peripherally to regulate energy homeostasis. Its large precursor protein, known as appetite-regulating hormone or motilin-related peptide, contains ghrelin and obestatin. Appetite-Regulating Hormone,GHRL Protein,Gastric MLTRP,Ghrelin Precursor,Ghrelin-Obestatin Preprohormone,Motilin-Related Peptide,Motilin-Related Peptide Precursor,Obestatin,PpMTLRP,Ppghrelin,Appetite Regulating Hormone,Ghrelin Obestatin Preprohormone,Motilin Related Peptide,Motilin Related Peptide Precursor,Peptide Precursor, Motilin-Related,Precursor, Ghrelin,Precursor, Motilin-Related Peptide
D019706 Excitatory Postsynaptic Potentials Depolarization of membrane potentials at the SYNAPTIC MEMBRANES of target neurons during neurotransmission. Excitatory postsynaptic potentials can singly or in summation reach the trigger threshold for ACTION POTENTIALS. EPSP,End Plate Potentials,Excitatory Postsynaptic Currents,Current, Excitatory Postsynaptic,Currents, Excitatory Postsynaptic,End Plate Potential,Excitatory Postsynaptic Current,Excitatory Postsynaptic Potential,Plate Potential, End,Plate Potentials, End,Postsynaptic Current, Excitatory,Postsynaptic Currents, Excitatory,Postsynaptic Potential, Excitatory,Postsynaptic Potentials, Excitatory,Potential, End Plate,Potential, Excitatory Postsynaptic,Potentials, End Plate,Potentials, Excitatory Postsynaptic
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

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