Neuregulin-1, the fetal endothelium, and brain damage in preterm newborns. 2010

Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
Pediatric Pulmonology and Neonatology, Hannover Medical School, Hannover, Germany.

OBJECTIVE To assess the potential role for Neuregulin-1 (NRG1) as a systemic endogenous protector in the setting of perinatal inflammatory brain damage. METHODS We measured NRG1-protein and mRNA levels in human umbilical venous endothelial cells (HUVECs) of different gestational ages at various durations of exposure to lipopolysaccharide (LPS). In parallel, we genotyped the donor individuals for SNP8NRG221533, a disease-related single nucleotide polymorphism in the 5' region upstream of the NRG1 sequence. Intracellular NRG1 localization was visualized by confocal microscopy. Furthermore we analyzed the relationship between SNP8NRG221533 genotype and neurodevelopmental outcome in children born preterm. RESULTS We observed a positive dose-response-relationship between NRG1-mRNA and intracellular protein levels with both advancing gestational age and duration of LPS exposure in HUVECs. The presence of allele C at the SNP8NRG221533 locus was associated with an increased cellular production of NRG1 in HUVECs, and with a significantly reduced risk for cerebral palsy and developmental delay in children born preterm. CONCLUSIONS In conclusion, our data indicate that gestational age, duration of LPS exposure, and the SNP8NRG221533 genotype affect NRG1 levels. Our results support the hypothesis that NRG1 may qualify as an endogenous protector during fetal development.

UI MeSH Term Description Entries
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D007234 Infant, Premature A human infant born before 37 weeks of GESTATION. Neonatal Prematurity,Premature Infants,Preterm Infants,Infant, Preterm,Infants, Premature,Infants, Preterm,Premature Infant,Prematurity, Neonatal,Preterm Infant
D007969 Leukomalacia, Periventricular Degeneration of white matter adjacent to the CEREBRAL VENTRICLES following cerebral hypoxia or BRAIN ISCHEMIA in neonates. The condition primarily affects white matter in the perfusion zone between superficial and deep branches of the MIDDLE CEREBRAL ARTERY. Clinical manifestations include VISION DISORDERS; CEREBRAL PALSY; PARAPLEGIA; SEIZURES; and cognitive disorders. (From Adams et al., Principles of Neurology, 6th ed, p1021; Joynt, Clinical Neurology, 1997, Ch4, pp30-1) Cystic Periventricular Leukomalacia,Encephalomalacia, Periventricular,Neonatal Cerebral Leukomalacia,Periventricular Leukomalacia,Leucomalacia, Periventricular,Cerebral Leukomalacia, Neonatal,Cerebral Leukomalacias, Neonatal,Cystic Periventricular Leukomalacias,Encephalomalacias, Periventricular,Leucomalacias, Periventricular,Leukomalacia, Cystic Periventricular,Leukomalacia, Neonatal Cerebral,Leukomalacias, Cystic Periventricular,Leukomalacias, Neonatal Cerebral,Leukomalacias, Periventricular,Neonatal Cerebral Leukomalacias,Periventricular Encephalomalacia,Periventricular Encephalomalacias,Periventricular Leucomalacia,Periventricular Leucomalacias,Periventricular Leukomalacia, Cystic,Periventricular Leukomalacias,Periventricular Leukomalacias, Cystic
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D005865 Gestational Age The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated from the onset of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization. It is also estimated to begin from fertilization, estrus, coitus, or artificial insemination. Embryologic Age,Fetal Maturity, Chronologic,Chronologic Fetal Maturity,Fetal Age,Maturity, Chronologic Fetal,Age, Embryologic,Age, Fetal,Age, Gestational,Ages, Embryologic,Ages, Fetal,Ages, Gestational,Embryologic Ages,Fetal Ages,Gestational Ages

Related Publications

Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
September 2012, American journal of obstetrics and gynecology,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
August 2004, Neurobiology of aging,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
January 2004, Biology of the neonate,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
August 2004, Early human development,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
January 2008, Neonatology,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
January 2018, Frontiers in pediatrics,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
November 2004, Placenta,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
June 2019, Open access Macedonian journal of medical sciences,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
February 2021, Journal of clinical medicine,
Insa Hoffmann, and Wolfgang Bueter, and Katja Zscheppang, and Maria-Jantje Brinkhaus, and Andrea Liese, and Stefan Riemke, and Thilo Dörk, and Olaf Dammann, and Christiane E L Dammann
April 2000, The Journal of pediatrics,
Copied contents to your clipboard!