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The synthesis of L-asparaginase in Escherichia coli W and E. coli K-12 was almost completely supressed if glucose was added at a concentration of 0.5 per cent to a growth medium. The level of L-asparaginase synthesis decreased by ca. 75 per cent as a result of cyamutations when the bacteria could not produce cyclo-3',5'-AMP (cAMP). Apparently, a decrease in the intracellular content of cAMP caused by glucose could not be the only factor inhibiting L-asparaginase synthesis. Lactate was found to stimulate L-asparaginase synthesis. Glucose caused the catabolite repression and catabolite inhibition of the components of a system involved in lactate transport. The inhibition of L-asparaginase synthesis by glucose seems to be due, at least partly, to the fact that it prevents the assimilation of lactate by the cells, as well as the utilization of some other compounds which stimulate synthesis of this enzyme.
M M Garaev, and
E I Golub
November 1969,
Biotechnology and bioengineering,
M M Garaev, and
E I Golub
January 1971,
The Journal of biological chemistry,
M M Garaev, and
E I Golub
October 1973,
Biochimica et biophysica acta,
M M Garaev, and
E I Golub
February 1977,
Applied and environmental microbiology,
M M Garaev, and
E I Golub
December 1968,
Journal of bacteriology,
M M Garaev, and
E I Golub
November 1977,
The Journal of biological chemistry,
M M Garaev, and
E I Golub
January 1974,
Folia microbiologica,
M M Garaev, and
E I Golub
February 1969,
Cancer research,
M M Garaev, and
E I Golub
April 1978,
Applied and environmental microbiology,
M M Garaev, and
E I Golub
August 1969,
Science (New York, N.Y.),
