BIOMAT Database Logo BIOMAT Database Logo

Solid phase indirect radioimmunoassay: standardization and applications in viral serology. 1977

L L Yung, and W Loh, and V ter Meulen

In solid phase indirect radioimmunoassay (IRIA) antiviral immunoglobulins (Ig) bind specifically to the viral antigen fixed onto wells of microtiter plates. Radioactively labeled (125I) anti-Ig can be used to detect these antiviral antibodies. The sensitivity of the IRIA depends on the amount of antigen in the microtiter wells and the concentration of the 125I-labeled anti-Ig used. To standardize the assay, a method of antigen titration in the IRIA was devised, using parainfluenza type 1 viruses as antigens. The IRIA provides a means to titrate viral antigens with different biologic activities and antibodies against them. A binding inhibition test (BIT) based on IRIA allows the antigenic analysis of different virus strains, as is demonstrated for two closely related parainfluenza type 1 virus strains.

UI MeSH Term Description Entries
D010222 Parainfluenza Virus 1, Human A species of RESPIROVIRUS also called hemadsorption virus 2 (HA2), which causes laryngotracheitis in humans, especially children. Hemadsorption Virus 2,Human parainfluenza virus 1,Para-Influenza Virus Type 1,Parainfluenza Virus Type 1,Para Influenza Virus Type 1
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D004306 Dose-Response Relationship, Immunologic A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell. Immunologic Dose-Response Relationship,Relationship, Immunologic Dose-Response,Dose Response Relationship, Immunologic,Dose-Response Relationships, Immunologic,Immunologic Dose Response Relationship,Immunologic Dose-Response Relationships,Relationship, Immunologic Dose Response,Relationships, Immunologic Dose-Response
D000888 Antibodies, Anti-Idiotypic Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D000956 Antigens, Viral Substances elaborated by viruses that have antigenic activity. Viral Antigen,Viral Antigens,Antigen, Viral

Related Publications

L L Yung, and W Loh, and V ter Meulen
Characterisation of anti-A monoclonals by liquid-phase and solid-phase serology and radioimmunoassay.
December 1987, Revue francaise de transfusion et immuno-hematologie,
L L Yung, and W Loh, and V ter Meulen
Rapid solid-phase radioimmunoassay for detection of equine infectious anemia viral antigen and antibodies: parameters involved in standardization.
October 1985, Journal of virological methods,
L L Yung, and W Loh, and V ter Meulen
Rubella serology by solid-phase radioimmunoassay: its potential for screening programmes.
January 1978, Clinical and experimental immunology,
L L Yung, and W Loh, and V ter Meulen
The principles and applications of solid-phase blood group serology.
January 1991, Transfusion medicine reviews,
L L Yung, and W Loh, and V ter Meulen
Solid phase radioimmunoassay.
February 1967, Nature,
L L Yung, and W Loh, and V ter Meulen
Characterisation of anti-B and anti-A + B monoclonals by liquid-phase and solid-phase serology and radioimmunoassay.
December 1987, Revue francaise de transfusion et immuno-hematologie,
L L Yung, and W Loh, and V ter Meulen
Completion of hemagglutination inhibition test by solid-phase radioimmunoassay test in routine diagnostic rubella serology.
August 1977, Medical biology,
L L Yung, and W Loh, and V ter Meulen
[The standardization of conjugates for the indirect solid-phase immunoenzyme analysis reaction].
April 1988, Zhurnal mikrobiologii, epidemiologii i immunobiologii,
L L Yung, and W Loh, and V ter Meulen
Detection of viral antigens by solid phase radioimmunoassay on polyethylene film.
April 1986, Journal of virological methods,
L L Yung, and W Loh, and V ter Meulen
Solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera.
November 1975, Journal of clinical microbiology,
Copied contents to your clipboard!