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Recent evidence suggests that positive and negative selection of thymocytes bearing alpha beta T-cell receptors occurs during the predominant double-positive (CD4+CD8+) stage. But the sequence or stage at which positive or negative selection occurs during thymocyte maturation has not been well defined. Here we use transgenic mice to show that the CD4+CD8+ stage might be further subdivided into CD3lo (low) and CD3in (intermediate) stages. The CD3in stage could represent T cells that have been positively selected, as this stage is dependent on the presence of the appropriate major histocompatibility complex restriction element. In addition, we use two different tolerizing antigens to show that negative selection may occur either before or after this CD3in stage.
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
June 1996,
Nature,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
March 1996,
European journal of immunology,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
November 2014,
Cell death & disease,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
January 1998,
Critical reviews in immunology,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
March 2007,
Blood,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
October 2013,
Science signaling,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
December 1989,
The Journal of experimental medicine,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
July 2000,
Nature,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
April 2002,
Proceedings of the National Academy of Sciences of the United States of America,
P S Ohashi, and
H Pircher, and
K Bürki, and
R M Zinkernagel, and
H Hengartner
August 1999,
Seminars in immunology,
