-transparent.png){kind=logo}
A new method of gibberellin A3 detection based on high-performance liquid chromatography has been elaborated. It combines high accuracy and low cost, and includes the following stages: fixation with liquid nitrogen; extraction of alcohol-soluble substances; purification with freezing, reextraction in ethyl acetate, and thin-layer chromatography; and detection with HPLC. Chromatographic conditions and methods of substance identification have been determined. The method allows the use of modest amounts of plant material (5-20 g), at which variability does not exceed 7%, and the total loss of substance is approximately 3%. The results of gibberellin A3 detection in buckwheat are presented.
E E Nefed'eva, and
N G Mazeĭ
January 1993,
Yao xue xue bao = Acta pharmaceutica Sinica,
E E Nefed'eva, and
N G Mazeĭ
October 1982,
Science (New York, N.Y.),
E E Nefed'eva, and
N G Mazeĭ
January 1989,
Chirality,
E E Nefed'eva, and
N G Mazeĭ
January 2011,
Zeitschrift fur Naturforschung. C, Journal of biosciences,
E E Nefed'eva, and
N G Mazeĭ
October 2012,
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences,
E E Nefed'eva, and
N G Mazeĭ
January 1990,
Methods in enzymology,
E E Nefed'eva, and
N G Mazeĭ
December 1983,
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
E E Nefed'eva, and
N G Mazeĭ
June 2005,
Journal of chromatography. A,
E E Nefed'eva, and
N G Mazeĭ
March 2010,
Journal of agricultural and food chemistry,
E E Nefed'eva, and
N G Mazeĭ
July 2011,
Food chemistry,
