Cloning of mouse ojoplano, a reticular cytoplasmic protein expressed during embryonic development. 2009

Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
Max Planck Institute for Molecular Genetics, Berlin, Germany.

Ojoplano (Opo) is a morphogenetic gene playing an important role during embryogenesis in medaka. This report focuses on the identification and characterization of the mouse Opo gene. We examined Opo expression by whole-mount in situ hybridization and in situ hybridization on sagittal sections during mouse embryogenesis. First expression in whole-mounts was detected at Theiler stages 15-17 (E 9.5-10.5dpc) as a spotted specific staining in migrating neural crest cells and in placodal structures. A complex expression pattern was observed in Theiler stage 22-23 (E 14.5dpc) in sagittal sections, including expression in skeletal structures (skull, vertebrae, ribs, bones of the locomotor system), in the nasal region, the heart and the eye. Fusion proteins revealed the localization of OPO within the cytoplasm with a reticular distribution that largely overlapped with the endoplasmic reticulum. Opo shows homology to human transcripts linked to a hereditary craniofacial malformation, orofacial cleft 1 (OFC1). The expression of mouse Opo in neural crest derivatives and skull elements further supports this link.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009024 Morphogenesis The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
D009432 Neural Crest The two longitudinal ridges along the PRIMITIVE STREAK appearing near the end of GASTRULATION during development of nervous system (NEURULATION). The ridges are formed by folding of NEURAL PLATE. Between the ridges is a neural groove which deepens as the fold become elevated. When the folds meet at midline, the groove becomes a closed tube, the NEURAL TUBE. Neural Crest Cells,Neural Fold,Neural Groove,Cell, Neural Crest,Cells, Neural Crest,Crest, Neural,Crests, Neural,Fold, Neural,Folds, Neural,Groove, Neural,Grooves, Neural,Neural Crest Cell,Neural Crests,Neural Folds,Neural Grooves
D009990 Oryzias The only genus in the family Oryziinae, order BELONIFORMES. Oryzias are egg-layers; other fish of the same order are livebearers. Oryzias are used extensively in testing carcinogens. Japanese Medaka,Killifish, Japanese,Medaka,Japanese Killifish,Japanese Rice Fish,Medaka Killifish,Oryzias latipes,Oryziinae,Killifish, Medaka,Medakas
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005136 Eye Proteins PROTEINS derived from TISSUES of the EYE. Proteins, Eye
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
January 2006, Gene expression patterns : GEP,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
February 1995, Molecular and biochemical parasitology,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
April 2005, Gene expression patterns : GEP,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
February 2005, Gene expression patterns : GEP,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
March 1993, Development (Cambridge, England),
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
October 2006, Gene expression patterns : GEP,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
March 2009, Gene expression patterns : GEP,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
November 1974, Biochemical and biophysical research communications,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
September 1996, The American journal of physiology,
Florian Mertes, and Juan-Ramon Martinez-Morales, and Tobias Nolden, and Ralf Spörle, and Joachim Wittbrodt, and Hans Lehrach, and Heinz Himmelbauer
February 1999, Plant & cell physiology,
Copied contents to your clipboard!