Expression of gap junctional protein connexin43 during 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis. 2009

Juan Xia, and Xiumei Liu, and Xiaoan Tao, and Yun Hong, and Xiaobing Chen, and Yaohui Dai, and Yulei Huang, and Bin Cheng
Department of Oral Medicine, Guanghua School of Stomatology and Institute of Stomatological Research, Sun Yat-Sen University,Guangzhou, Guangdong, People's Republic of China.

Oral carcinogenesis is a multistep process and requires accumulation and interplay of a series of molecular genetic events. Gap junctions are intercellular channels composed of connexin subunits that mediate cell-cell communication. The disfunctions of gap junctions are believed to be associated with cancer development. We therefore investigated the expression of connexin (Cx)43, one of the major connexins in oral epithelia, during 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis. By immunohistochemistry, Cx43 expression was observed mainly in the cell membrane in normal rat oral epithelia. It was weak in the basal cell layer, increased in the stratum spinosum and stratum granulosum, and negative in the stratum corneum of normal epithelia. Throughout the course of carcinogenesis, both Cx43 immunostained area and mean intensity decreased with significant difference among various histopathological groups (P < 0.05). In cancerous oral epithelia cytoplasmic staining could be observed. However, Cx43 mRNA level showed no significant difference in the progress of oral carcinogenesis (P > 0.05) and without correlation to Cx43 protein immunostained area and mean intensity. Our results indicated that downregulation of Cx43 might be an early event during oral carcinogenesis, which could be a biomarker for early changes in oral malignant transformation.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297 Male Males
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014062 Tongue Neoplasms Tumors or cancer of the TONGUE. Cancer of Tongue,Tongue Cancer,Cancer of the Tongue,Neoplasms, Tongue,Cancer, Tongue,Cancers, Tongue,Neoplasm, Tongue,Tongue Cancers,Tongue Neoplasm
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D015112 4-Nitroquinoline-1-oxide A potent mutagen and carcinogen. This compound and its metabolite 4-HYDROXYAMINOQUINOLINE-1-OXIDE bind to nucleic acids. It inactivates bacteria but not bacteriophage. 4-Nitroquinoline-N-oxide,4 Nitroquinoline 1 oxide,4 Nitroquinoline N oxide
D015972 Gene Expression Regulation, Neoplastic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. Neoplastic Gene Expression Regulation,Regulation of Gene Expression, Neoplastic,Regulation, Gene Expression, Neoplastic
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats

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