Biomaterial Database

Surface associated glycoproteins from Toxocara canis larval parasites. 1990

R M Maizels, and A P Page

Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.

The surface of infective larvae of Toxocara canis, the dog ascarid nematode, reveals relatively few exposed surface proteins which can be recovered in soluble form. The major components identified by surface labelling have molecular weights of 32, 55, 70 and 120 kilodaltons (kDa), and are all significantly glycosylated. All are recognised by the immune response in definitive (canine) and paratenic (murine or human) hosts. Expression of these antigens on the parasite surface begins after the larvae hatch from infective ova in vitro, and presumably in vivo. Each of these molecules may also be found in the set of secreted (ES) glycoconjugates released by larval parasites cultivated in vitro, and currently available biochemical and functional data on the surface/secreted ES glycoproteins are presented. Analysis with monoclonal antibodies (MAbs) confirms the identity of surface and ES molecules, and these MAbs show differing patterns of binding to the epicuticle, the cuticular matrix and to the oral orifice. Alternative mechanisms for antigen synthesis, insertion into the cuticle and export from the parasite are discussed.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007814	Larva	Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.	Maggots,Tadpoles,Larvae,Maggot,Tadpole
D008562	Membrane Glycoproteins	Glycoproteins found on the membrane or surface of cells.	Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000947	Antigens, Helminth	Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.	Helminth Antigens
D014119	Toxocara	A genus of ascarid nematodes commonly parasitic in the intestines of cats and dogs.	Toxocaras
D015801	Helminth Proteins	Proteins found in any species of helminth.	Helminth Protein,Protein, Helminth,Proteins, Helminth