Biomaterial Database

Rat DARPP-32: cloning, sequencing, and characterization of the cDNA. 1990

M E Ehrlich, and T Kurihara, and P Greengard

Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021.

DARPP-32, a substrate for cyclic AMP-dependent protein kinase, is highly enriched in the caudate nucleus. In the present study, the cDNA for rat DARPP-32 was isolated and characterized. When compared to the coding region of bovine DARPP-32 cDNA, there was 86% identity at the nucleotide level, and 84% identity at the amino acid level. The homology in the region previously noted to be similar to phosphatase inhibitor-1 remained intact. There were also 3'-untranslated regions that were highly conserved. The DARPP-32 mRNA, which appeared as a doublet, was widely distributed in the central nervous system and was highly enriched in the striatum. Southern blot analysis revealed a simple hybridization pattern, consistent with the presence of a single gene coding for rat DARPP-32. Unilateral destruction of the nigrostriatal dopaminergic pathway by injection of 6-hydroxydopamine (6-OHDA) into the substantia nigra did not alter the striatal steady state levels of DARPP-32 mRNA.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009419	Nerve Tissue Proteins		Proteins, Nerve Tissue,Tissue Proteins, Nerve
D010750	Phosphoproteins		Phosphoprotein
D011061	Poly A	A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.	Adenine Polynucleotides,Polyadenylic Acids,Poly(rA),Polynucleotides, Adenine
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D003342	Corpus Striatum	Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.	Lenticular Nucleus,Lentiform Nucleus,Lentiform Nuclei,Nucleus Lentiformis,Lentiformis, Nucleus,Nuclei, Lentiform,Nucleus, Lenticular,Nucleus, Lentiform,Striatum, Corpus
D005786	Gene Expression Regulation	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.	Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006892	Hydroxydopamines	Dopamines with a hydroxy group substituted in one or more positions.	Hydroxydopamine
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia