BIOMAT Database Logo BIOMAT Database Logo

Generation of neutralizing monoclonal antibodies against Enterovirus 71 using synthetic peptides. 2009

Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Beijing Vigoo Biologicals Co., Ltd, 4 South San Jian Fan, Chaoyang District, Beijing 100024, China.

Enterovirus 71 (EV71) has led to recent outbreaks of hand, foot and mouth disease (HFMD) in China, resulting in high mortality. In this study, several monoclonal antibodies were generated by immunizing mice with two synthetic peptides, SP55 and SP70, containing amino acids 163-177 and 208-222 of VP1. The specificities of the anti-EV71 peptide monoclonal antibodies were confirmed by Western blot analysis and immunocytochemistry against EV71 virus. Most importantly, we have identified a monoclonal antibody, clone 22A12, which shows strong neutralizing activity against EV71 in an in vitro neutralization assay. Because there is no vaccine available and treatment is very limited, mouse anti-EV71 monoclonal antibody, clone 22A12, could be a promising candidate to be humanized and used for treatment of EV71 infection.

UI MeSH Term Description Entries
D009500 Neutralization Tests The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). Neutralization Test,Test, Neutralization,Tests, Neutralization
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D003384 Coxsackievirus Infections A heterogeneous group of infections produced by coxsackieviruses, including HERPANGINA, aseptic meningitis (MENINGITIS, ASEPTIC), a common-cold-like syndrome, a non-paralytic poliomyelitis-like syndrome, epidemic pleurodynia (PLEURODYNIA, EPIDEMIC) and a serious MYOCARDITIS. Coxsackie Virus Infections,Infections, Coxsackie Virus,Infections, Coxsackievirus,Coxsackie Virus Infection,Coxsackievirus Infection
D006232 Hand, Foot and Mouth Disease A mild, highly infectious viral disease of children, characterized by vesicular lesions in the mouth and on the hands and feet. It is caused by coxsackieviruses A. Hand Foot and Mouth Disease,Hand, Foot, and Mouth Disease,Hand, Foot, Mouth Disease
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D014765 Viral Vaccines Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease. Viral Vaccine,Vaccine, Viral,Vaccines, Viral
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
[Preparation of pan-neutralizing mouse monoclonal antibodies against enterovirus 71 and coxsackievirus A16].
July 2021, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides.
April 2007, Virus research,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Characterization of neutralizing antibodies to bovine enterovirus elicited by synthetic peptides.
January 1992, Archives of virology,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Short-term dynamic changes in neutralizing antibodies against enterovirus 71 after vaccination.
July 2020, Human vaccines & immunotherapeutics,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Neutralizing antibodies to enterovirus 71 in Belém, Brazil.
January 2002, Memorias do Instituto Oswaldo Cruz,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Monoclonal antibodies for diagnosis of enterovirus 71.
December 2013, Monoclonal antibodies in immunodiagnosis and immunotherapy,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Passive protection against lethal enterovirus 71 infection in newborn mice by neutralizing antibodies elicited by a synthetic peptide.
September 2007, Microbes and infection,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Generation and characterization of a protective mouse monoclonal antibody against human enterovirus 71.
September 2015, Applied microbiology and biotechnology,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Generation of neutralizing and non-neutralizing monoclonal antibodies against H7N9 influenza virus.
January 2020, Emerging microbes & infections,
Xiuling Li, and Chunming Mao, and Shujun Ma, and Xiaoqing Wang, and Zhe Sun, and Yao Yi, and Minzhuo Guo, and Xinliang Shen, and Le Sun, and Shengli Bi
Neutralizing antibodies can initiate genome release from human enterovirus 71.
February 2014, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!