Determination of full-length cDNA nucleotide sequence of equine carbonic anhydrase VI and its expression in various tissues. 2009

Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
Research Institute of Biosciences, Azabu University, Sagamihara, Kanagawa, Japan. ochiaih@azabu-u.ac.jp

A full-length cDNA clone of an equine carbonic anhydrase (CA)-VI was obtained from the equine parotid gland. The cDNA sequence was 1338 bp long and was predicted to encode a 319 amino acid polypeptide with a putative signal peptide of 18 amino acids. The deduced amino acid sequence of mature CA-VI showed the similarity of 70% to those of other mammalians reported. Westernblot analysis using anti-horse CA-VI peptide detected the single band in parotid gland, and the band reduced its size by treatment with N-glycosidase F. Additionally, CA-VI protein expression was confirmed in submandicular gland and weakly in liver. In contrast, RT-PCR analysis revealed signals in the digestive tract including duodenum, jejunum, ileum, cecum and colon as well as the salivary glands. In addition, certain signals were detected in testis, thyroid gland and liver, but not in nerve tissue, skeletal muscle, spleen or lymph node.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008198 Lymph Nodes They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system. Lymph Node,Node, Lymph,Nodes, Lymph
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002256 Carbonic Anhydrases A family of zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide. They play an important role in the transport of CARBON DIOXIDE from the tissues to the LUNG. EC 4.2.1.1. Carbonate Dehydratase,Carbonic Anhydrase,Anhydrases, Carbonic,Dehydratase, Carbonate
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006736 Horses Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest. Equus caballus,Equus przewalskii,Horse, Domestic,Domestic Horse,Domestic Horses,Horse,Horses, Domestic
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
June 2003, DNA sequence : the journal of DNA sequencing and mapping,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
June 1990, Nucleic acids research,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
June 1987, Nucleic acids research,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
January 1990, Plant physiology,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
June 1998, American journal of veterinary research,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
May 2004, Veterinary immunology and immunopathology,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
January 1995, Plant physiology,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
January 1991, Biochemistry,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
December 1991, Gene,
Hideharu Ochiai, and Nobuyuki Kanemaki, and Shino Kamoshida, and Masaru Murakami, and Nobutsune Ichihara, and Masao Asari, and Toshiho Nishita
February 1989, Biochemical genetics,
Copied contents to your clipboard!