BIOMAT Database Logo BIOMAT Database Logo

Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM. 1990

B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
National Bacteriological Laboratory, Stockholm, Sweden.

IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR. IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies. Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the "one-way" crossing described earlier.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D007723 Korea Former kingdom, located on Korea Peninsula between Sea of Japan and Yellow Sea on east coast of Asia. In 1948, the kingdom ceased and two independent countries were formed, divided by the 38th parallel.
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D004812 Epidemiologic Methods Research techniques that focus on study designs and data gathering methods in human and animal populations. Epidemiologic Method,Epidemiological Methods,Methods, Epidemiologic,Epidemiological Method,Method, Epidemiologic,Method, Epidemiological,Methods, Epidemiological
D005060 Europe The continent north of AFRICA, west of ASIA and east of the ATLANTIC OCEAN. Northern Europe,Southern Europe,Western Europe
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D006476 Orthohantavirus A genus of the family BUNYAVIRIDAE causing HANTAVIRUS INFECTIONS, first identified during the Korean war. Infection is found primarily in rodents and humans. Transmission does not appear to involve arthropods. HANTAAN VIRUS is the type species. Dobrava-Belgrade Virus,Hantavirus,Andes Hantavirus,Andes Virus,Andes Hantaviruses,Dobrava Belgrade Virus,Hantavirus, Andes,Hantaviruses,Hantaviruses, Andes,Orthohantaviruses
D006480 Hemorrhagic Fever with Renal Syndrome An acute febrile disease occurring predominately in Asia. It is characterized by fever, prostration, vomiting, hemorrhagic phenonema, shock, and renal failure. It is caused by any one of several closely related species of the genus ORTHOHANTAVIRUS. The most severe form is caused by HANTAAN VIRUS whose natural host is the rodent Apodemus agrarius. Milder forms are caused by SEOUL VIRUS and transmitted by the rodents Rattus rattus and R. norvegicus, and the PUUMALA VIRUS with transmission by Clethrionomys galreolus. HFRS,Hemorrhagic Fever, Epidemic,Hemorrhagic Fever, Korean,Hemorrhagic Nephroso-Nephritis,Nephropathia Epidemica,Epidemic Hemorrhagic Fever,Epidemic Hemorrhagic Fevers,Hemorrhagic Fevers, Epidemic,Hemorrhagic Nephroso Nephritis,Hemorrhagic Nephroso-Nephritides,Korean Hemorrhagic Fever,Nephroso-Nephritides, Hemorrhagic,Nephroso-Nephritis, Hemorrhagic

Related Publications

B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Specific Puumala IgG virus half a century after haemorrhagic fever with renal syndrome.
July 1991, Lancet (London, England),
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Enzyme immuno assay for the detection of virus specific IgG and IgM antibody in patients with haemorrhagic fever with renal syndrome.
January 1988, Archives of virology,
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Prevalence of serum IgG antibodies to Puumala virus (haemorrhagic fever with renal syndrome) in northern Sweden.
August 1994, Epidemiology and infection,
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Haemorrhagic fever with renal syndrome caused by Puumala virus: evaluation of the risk for cataract formation.
January 1998, Eye (London, England),
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
ELISA for detection of IgM and IgG antibodies to sandfly fever Sicilian virus.
January 1991, Research in virology,
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV-specific human IgG and IgM antibodies by indirect ELISA.
August 2015, Virology journal,
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
ELISA for the detection of specific IgM and IgG in human leptospirosis.
February 1985, Journal of general microbiology,
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection.
January 2022, The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases,
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Insufficient efficacy and safety of intravenous ribavirin in treatment of haemorrhagic fever with renal syndrome caused by Puumala virus.
July 2017, Infectious diseases (London, England),
B Niklasson, and E Tkachenko, and A P Ivanov, and G van der Groen, and D Wiger, and H K Andersen, and J LeDuc, and T Kjelsson, and K Nyström
Analytical Performance of the RIDASCREEN® Hantavirus Puumala IgG/IgM ELISA Assay.
February 2020, Viruses,
Copied contents to your clipboard!