Biomaterial Database

[Stimulatory effects of Bacteroides gingivalis fimbriae on production of fibroblasts-derived thymocyte-activating factor (FTAF) by human gingival fibroblasts]. 1990

K Hirose

Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.

Bacteroides gingivalis (B. gingivalis) is isolated frequently from subgingival plaques of adult periodontal patients. B. gingivalis is a gram-negative anaerobic organism which has fimbriae on its cell surface. In the present study, B. gingivalis fimbriae were examined for their ability to adhere to human gingival fibroblasts (Gin-1), and to stimulate fibroblast-derived thymocyte-activating factor (FTAF) production by Gin-1 cells. The ability of the fimbriae to bind specifically to Gin-1 cells was clearly shown by competition assay between 125I-labeled and unlabeled fimbriae. Significant stimulatory effect of the fimbriae on FTAF production was observed, when the fimbriae were added to Gin-1 cells at a dose of 1 microgram/ml, and this stimulation was observed as early as 24 hr after addition of fimbriae to the cells. It was verified by a spleen cell mitogenic assay for the fimbriae that the stimulatory effect was not due to lipopolysaccharide (LPS) contamination of the fimbriae preparation. The FTAF activity was inhibited about 50% by recombinant human interleukin-1 (IL-1) beta antiserum but not by recombinant human IL-1 alpha antiserum. Therefore, the present study suggests that B. gingivalis fimbriae may play a functional role in the pathogenesis of adult periodontal disease induced by the microorganism.

UI	MeSH Term	Description	Entries
D007375	Interleukin-1	A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.	IL-1,Lymphocyte-Activating Factor,Epidermal Cell Derived Thymocyte-Activating Factor,Interleukin I,Macrophage Cell Factor,T Helper Factor,Epidermal Cell Derived Thymocyte Activating Factor,Interleukin 1,Lymphocyte Activating Factor
D010861	Fimbriae, Bacterial	Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).	Bacterial Fimbriae,Bacterial Pili,Common Fimbriae,Common Pili,Pili, Bacterial,Pili, Common,Bacterial Fimbria,Bacterial Pilus,Common Fimbria,Common Pilus,Fimbria, Bacterial,Pilus, Bacterial,Fimbria, Common,Fimbriae, Common,Pilus, Common
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D005881	Gingiva	Oral tissue surrounding and attached to TEETH.	Gums,Interdental Papilla,Papilla, Interdental,Gum
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001422	Bacterial Adhesion	Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.	Adhesion, Bacterial,Adhesions, Bacterial,Bacterial Adhesions
D016207	Cytokines	Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.	Cytokine
D016966	Porphyromonas gingivalis	A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium produces a cell-bound, oxygen-sensitive collagenase and is isolated from the human mouth.	Bacteroides gingivalis