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The in vitro unscheduled DNA synthesis (UDS) assay has been evaluated in rat primary lung cells with known genotoxicants. The autoradiographic method was employed to detect UDS in both alveolar macrophages and primary pulmonary cells. Data of a time course study revealed that a high radioactive labeling of DNA repair was achieved after a 16-h incubation with [3H]thymidine. Coupled with low serum (1%), hydroxyurea at the concentration of 20 mM inhibited regular DNA synthesis in primary lung cells in a satisfactory manner (81-88% inhibition). With this protocol, a dose-related increase in UDS was induced by N-methyl-N'-nitro-N-nitrosoguanidine and 2-aminoanthracene in both rat alveolar macrophages and primary lung cells. The results suggest that primary rat lung cells in culture possess DNA-repair ability and that the UDS assay may be useful for assessing the pulmonary genotoxic effect of chemicals in this cell system.
W Z Whong, and
J D Stewart, and
T Ong
June 1989,
Mutation research,
W Z Whong, and
J D Stewart, and
T Ong
January 1995,
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W Z Whong, and
J D Stewart, and
T Ong
October 1984,
Mutation research,
W Z Whong, and
J D Stewart, and
T Ong
August 1985,
Mutation research,
W Z Whong, and
J D Stewart, and
T Ong
January 1986,
Environmental mutagenesis,
W Z Whong, and
J D Stewart, and
T Ong
December 1985,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
W Z Whong, and
J D Stewart, and
T Ong
June 1989,
Mutation research,
W Z Whong, and
J D Stewart, and
T Ong
January 2000,
Environmental and molecular mutagenesis,
W Z Whong, and
J D Stewart, and
T Ong
January 1995,
Environmental and molecular mutagenesis,
W Z Whong, and
J D Stewart, and
T Ong
November 1987,
Mutagenesis,
