BIOMAT Database Logo BIOMAT Database Logo

Novel 3'-O-fluorescently modified nucleotides for reversible termination of DNA synthesis. 2010

Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Life Sciences Research Division, Korea Institute of Science and Technology, Cheongryang, Seoul, Korea.

UI MeSH Term Description Entries
D009711 Nucleotides The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed) Nucleotide
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004259 DNA-Directed DNA Polymerase DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair. DNA Polymerase,DNA Polymerases,DNA-Dependent DNA Polymerases,DNA Polymerase N3,DNA Dependent DNA Polymerases,DNA Directed DNA Polymerase,DNA Polymerase, DNA-Directed,DNA Polymerases, DNA-Dependent,Polymerase N3, DNA,Polymerase, DNA,Polymerase, DNA-Directed DNA,Polymerases, DNA,Polymerases, DNA-Dependent DNA
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D014468 Umbelliferones 7-Hydroxycoumarins. Substances present in many plants, especially umbelliferae. Umbelliferones are used in sunscreen preparations and may be mutagenic. Their derivatives are used in liver therapy, as reagents, plant growth factors, sunscreens, insecticides, parasiticides, choleretics, spasmolytics, etc. 7-Hydroxycoumarins,Hydrangin,Skimmetin,7 Hydroxycoumarins
D019032 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis. Laser Desorption-Ionization Mass Spectrometry, Matrix-Assisted,MALD-MS,MALDI,Mass Spectrometry, Matrix-Assisted Laser Desorption-Ionization,Mass Spectroscopy, Matrix-Assisted Laser Desorption-Ionization,Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry,Spectroscopy, Mass, Matrix-Assisted Laser Desorption-Ionization,MALDI-MS,MS-MALD,SELDI-TOF-MS,Surface Enhanced Laser Desorption Ionization Mass Spectrometry,Laser Desorption Ionization Mass Spectrometry, Matrix Assisted,MALDI MS,Mass Spectrometry, Matrix Assisted Laser Desorption Ionization,Mass Spectroscopy, Matrix Assisted Laser Desorption Ionization,Matrix Assisted Laser Desorption Ionization Mass Spectrometry

Related Publications

Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates.
October 1994, Nucleic acids research,
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications.
January 2008, Nucleic acids symposium series (2004),
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
A diversity oriented synthesis of 3'-O-modified nucleoside triphosphates for DNA 'sequencing by synthesis'.
August 2006, Bioorganic & medicinal chemistry letters,
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
A novel procedure for the synthesis of 2'-O-alkyl nucleotides.
December 1972, Biochemistry,
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Termination of DNA synthesis by N6-alkylated, not 3'-O-alkylated, photocleavable 2'-deoxyadenosine triphosphates.
January 2007, Nucleic acids research,
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing.
February 2016, Chemical communications (Cambridge, England),
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Template-directed synthesis in 3'- and 5'-direction with reversible termination.
August 2012, Angewandte Chemie (International ed. in English),
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Versatile linker chemistry for synthesis of 3'-modified DNA.
January 1997, Bioconjugate chemistry,
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
Enzymatic Synthesis of Designer DNA Using Cyclic Reversible Termination and a Universal Template.
February 2020, ACS synthetic biology,
Taek-Soo Kim, and Da-Rae Kim, and Hee-Chul Ahn, and Dongyun Shin, and Dae-Ro Ahn
A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis.
March 2008, Nucleic acids research,
Copied contents to your clipboard!