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Visualization and identification of IL-7 producing cells in reporter mice. 2009
Renata I Mazzucchelli, and
Søren Warming, and
Scott M Lawrence, and
Masaru Ishii, and
Mehrnoosh Abshari, and
A Valance Washington, and
Lionel Feigenbaum, and
Andrew C Warner, and
Davis J Sims, and
Wen Qing Li, and
Julie A Hixon, and
Daniel H D Gray, and
Benjamin E Rich, and
Matthew Morrow, and
Miriam R Anver, and
James Cherry, and
Dieter Naf, and
Lawrence R Sternberg, and
Daniel W McVicar, and
Andrew G Farr, and
Ronald N Germain, and
Keith Rogers, and
Nancy A Jenkins, and
Neal G Copeland, and
Scott K Durum
Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, NIH, Frederick, MD, USA.
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.
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Mehrnoosh Abshari, and
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Lionel Feigenbaum, and
Andrew C Warner, and
Davis J Sims, and
Wen Qing Li, and
Julie A Hixon, and
Daniel H D Gray, and
Benjamin E Rich, and
Matthew Morrow, and
Miriam R Anver, and
James Cherry, and
Dieter Naf, and
Lawrence R Sternberg, and
Daniel W McVicar, and
Andrew G Farr, and
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