Human ribosomal protein S13 promotes gastric cancer growth through down-regulating p27(Kip1). 2011

Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.

Our previous works revealed that human ribosomal protein S13 (RPS13) was up-regulated in multidrug-resistant gastric cancer cells and overexpression of RPS13 could protect gastric cancer cells from drug-induced apoptosis. The present study was designed to explore the role of RPS13 in tumorigenesis and development of gastric cancer. The expression of RPS13 in gastric cancer tissues and normal gastric mucosa was evaluated by immunohistochemical staining and Western blot analysis. It was found RPS13 was expressed at a higher level in gastric cancer tissues than that in normal gastric mucosa. RPS13 was then genetically overexpressed in gastric cancer cells or knocked down by RNA interference. It was demonstrated that up-regulation of RPS13 accelerated the growth, enhanced in vitro colony forming and soft agar cologenic ability and promoted in vivo tumour formation potential of gastric cancer cells. Meanwhile, down-regulation of RPS13 in gastric cancer cells resulted in complete opposite effects. Moreover, overexpression of RPS13 could promote G1 to S phase transition whereas knocking down of RPS13 led to G1 arrest of gastric cancer cells. It was further demonstrated that RPS13 down-regulated p27(kip1) expression and CDK2 kinase activity but did not change the expression of cyclin D, cyclin E, CDK2, CDK4 and p16(INK4A). Taken together, these data indicate that RPS13 could promote the growth and cell cycle progression of gastric cancer cells at least through inhibiting p27(kip1) expression.

UI MeSH Term Description Entries
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008819 Mice, Nude Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses. Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D005753 Gastric Mucosa Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones. Cardiac Glands,Gastric Glands,Pyloric Glands,Cardiac Gland,Gastric Gland,Gastric Mucosas,Gland, Cardiac,Gland, Gastric,Gland, Pyloric,Glands, Cardiac,Glands, Gastric,Glands, Pyloric,Mucosa, Gastric,Mucosas, Gastric,Pyloric Gland
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012269 Ribosomal Proteins Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits. Proteins, Ribosomal,Ribosomal Protein,Protein, Ribosomal
D013274 Stomach Neoplasms Tumors or cancer of the STOMACH. Cancer of Stomach,Gastric Cancer,Gastric Neoplasms,Stomach Cancer,Cancer of the Stomach,Gastric Cancer, Familial Diffuse,Neoplasms, Gastric,Neoplasms, Stomach,Cancer, Gastric,Cancer, Stomach,Cancers, Gastric,Cancers, Stomach,Gastric Cancers,Gastric Neoplasm,Neoplasm, Gastric,Neoplasm, Stomach,Stomach Cancers,Stomach Neoplasm
D015536 Down-Regulation A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Down-Regulation,Down-Regulation (Physiology),Downregulation,Down Regulation,Down-Regulation, Receptor
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis

Related Publications

Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
September 2021, Aging,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
January 2002, Cell cycle (Georgetown, Tex.),
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
April 2012, Molecular cancer therapeutics,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
April 1999, The Journal of pathology,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
July 2001, The Biochemical journal,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
March 2010, Biochemical and biophysical research communications,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
January 2016, Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
January 2008, PloS one,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
January 2015, OncoTargets and therapy,
Xueyan Guo, and Yongquan Shi, and Yawen Gou, and Jipeng Li, and Shuang Han, and Yanqi Zhang, and Jianhua Huo, and Xiaoxuan Ning, and Li Sun, and Yu Chen, and Shiren Sun, and Daiming Fan
January 2011, The Journal of biological chemistry,
Copied contents to your clipboard!