BIOMAT Database Logo BIOMAT Database Logo

Effect of L-methionine on 2-carboxybenzaldehyde reductase induction by phenobarbital in primary cultures of rat hepatocytes. 1991

K Tonda, and M Hirata
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.

Effects of phenobarbital (PB) and L-methionine on 2-carboxybenzaldehyde (CBA) reductase in rat hepatocyte primary culture were examined. Inclusion of PB in the culture medium markedly enhanced the CBA reductase activity while L-methionine, which elevates the cellular glutathione (GSH) level, suppressed the stimulatory effect of PB. This suppression, though less pronounced, was also found with other precursors of GSH biosynthesis. GSH-depletors, buthionine sulfoximine (BSO) or diethylmaleate (DEM), enhanced the CBA reductase activity suggesting that GSH plays an important role in enzyme induction.

UI MeSH Term Description Entries
D008297 Male Males
D008715 Methionine A sulfur-containing essential L-amino acid that is important in many body functions. L-Methionine,Liquimeth,Methionine, L-Isomer,Pedameth,L-Isomer Methionine,Methionine, L Isomer
D010634 Phenobarbital A barbituric acid derivative that acts as a nonselective central nervous system depressant. It potentiates GAMMA-AMINOBUTYRIC ACID action on GABA-A RECEPTORS, and modulates chloride currents through receptor channels. It also inhibits glutamate induced depolarizations. Phenemal,Phenobarbitone,Phenylbarbital,Gardenal,Hysteps,Luminal,Phenobarbital Sodium,Phenobarbital, Monosodium Salt,Phenylethylbarbituric Acid,Acid, Phenylethylbarbituric,Monosodium Salt Phenobarbital,Sodium, Phenobarbital
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003513 Cycloheximide Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis. Actidione,Cicloheximide
D003540 Cystathionine Sulfur-containing amino acid formed as an intermediate in the conversion of METHIONINE to CYSTEINE.
D003609 Dactinomycin A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015) Actinomycin,Actinomycin D,Meractinomycin,Cosmegen,Cosmegen Lyovac,Lyovac-Cosmegen,Lyovac Cosmegen,Lyovac, Cosmegen,LyovacCosmegen
D004790 Enzyme Induction An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis. Induction, Enzyme
D005978 Glutathione A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine

Related Publications

K Tonda, and M Hirata
Critical role of extracellular matrix on induction by phenobarbital of cytochrome P450 2B1/2 in primary cultures of adult rat hepatocytes.
December 1995, Laboratory investigation; a journal of technical methods and pathology,
K Tonda, and M Hirata
Cytochrome P-450 induction by phenobarbital and 3-methylcholanthrene in primary cultures of hepatocytes.
September 1976, Science (New York, N.Y.),
K Tonda, and M Hirata
Baculovirus vectors repress phenobarbital-mediated gene induction and stimulate cytokine expression in primary cultures of rat hepatocytes.
August 2000, Gene therapy,
K Tonda, and M Hirata
Differentiated induction of cytochrome P450b/e and P450p mRNAs by dose of phenobarbital in primary cultures of adult rat hepatocytes.
October 1990, Molecular pharmacology,
K Tonda, and M Hirata
Characterization of a pretranscriptional pathway for induction by phenobarbital of cytochrome P450 3A23 in primary cultures of adult rat hepatocytes.
June 1997, Archives of biochemistry and biophysics,
K Tonda, and M Hirata
N-Nitrosodiethylamine genotoxicity in primary rat hepatocytes: effects of cytochrome P450 induction by phenobarbital.
October 2011, Toxicology letters,
K Tonda, and M Hirata
Induction of DNA damage by menadione (2-methyl-1,4-naphthoquinone) in primary cultures of rat hepatocytes.
June 1984, Biochemical pharmacology,
K Tonda, and M Hirata
Effect of arsenite on induction of CYP1A, CYP2B, and CYP3A in primary cultures of rat hepatocytes.
May 1999, Toxicology and applied pharmacology,
K Tonda, and M Hirata
Effect of exogenous S-adenosyl-L-methionine on phosphatidylcholine synthesis by isolated rat hepatocytes.
May 1984, Biochemical pharmacology,
K Tonda, and M Hirata
Induction of cell proliferation by cyclosporine A in primary cultures of rat hepatocytes.
February 2001, Biochemical pharmacology,
Copied contents to your clipboard!