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Study of interaction of ceruloplasmin, lactoferrin, and myeloperoxidase by photon correlation spectroscopy. 2009

A V Sokolov, and V N Prozorovskii, and V B Vasilyev
Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia. biochem@nm.ru

In this work, the diameters of protein complexes formed upon interaction of ceruloplasmin (CP) with lactoferrin (LF) and myeloperoxidase (MPO) were determined. Gage dependence of the diameter of protein particles (myoglobin, albumin, LF, CP, MPO, aldolase, ferritin) on their molecular mass logarithm was calculated. The diameter of a complex formed upon mixing CP and LF was 8.4 nm, which is in line with the radius of gyration obtained previously when the 1CP-1LF complex was studied by small-angle X-ray scattering. The diameter of a complex formed upon interaction of CP with MPO is 9.8 nm, corresponding to the stoichiometry 2CP : 1MPO. The diameter of a complex formed when LF is added to the 2CP-1MPO complex is 10.7 nm. The latter is consistent with the notion of a pentameric structure 2LF-2CP-1MPO with molecular mass of about 585 kDa.

UI MeSH Term Description Entries
D007781 Lactoferrin An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion. Lactotransferrin
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009195 Peroxidase A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7. Myeloperoxidase,Hemi-Myeloperoxidase,Hemi Myeloperoxidase
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002570 Ceruloplasmin A multi-copper blood FERROXIDASE involved in iron and copper homeostasis and inflammation. Caeruloplasmin,Ferroxidase,Ceruloplasmin Ferroxidase,Ceruloplasmin Oxidase,Ferroxidase I,alpha(2)-Ceruloplasmin,Ferroxidase, Ceruloplasmin,Oxidase, Ceruloplasmin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014961 X-Ray Diffraction The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions
D056951 Photoelectron Spectroscopy The study of the energy of electrons ejected from matter by the photoelectric effect, i.e., as a direct result of absorption of energy from electromagnetic radiation. As the energies of the electrons are characteristic of a specific element, the measurement of the energy of these electrons is a technique used to determine the chemical composition of surfaces. Photoelectron Spectrometry,Electron Spectroscopy for Chemical Analysis,Photoelectron Emission Spectroscopy,Photoemission Spectroscopy,Ultra-Violet Photoelectron Spectroscopy,Ultraviolet Photoelectron Spectroscopy,X-Ray Photoelectron Spectroscopy,Xray Photoelectron Spectroscopy,Emission Spectroscopies, Photoelectron,Emission Spectroscopy, Photoelectron,Photoelectron Spectroscopy, Ultra-Violet,Photoelectron Spectroscopy, Ultraviolet,Photoelectron Spectroscopy, X-Ray,Photoelectron Spectroscopy, Xray,Spectrometry, Photoelectron,Spectroscopy, Photoelectron,Spectroscopy, Photoelectron Emission,Spectroscopy, Photoemission,Spectroscopy, Ultra-Violet Photoelectron,Spectroscopy, Ultraviolet Photoelectron,Spectroscopy, X-Ray Photoelectron,Spectroscopy, Xray Photoelectron,Ultra Violet Photoelectron Spectroscopy,X Ray Photoelectron Spectroscopy

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