Biomaterial Database

Differentiation of Fusarium from Aspergillus species by colorimetric in situ hybridization in formalin-fixed, paraffin-embedded tissue sections using dual fluorogenic-labeled LNA probes. 2009

Kathleen T Montone

Dept of Pathology and Laboratory Medicine, Hospital of University of Pennsylvania, 3400 Spruce St, 6 Founders, Philadelphia, PA 19104, USA.

Fusarium and Aspergillus are 2 genera of fungal pathogens that can result in devastating disease particularly in immunosuppressed hosts. In tissue sections, these organisms can be extremely difficult to distinguish from one another. To differentiate between these 2 pathogens, a rapid (<3 hours) ribosomal RNA (rRNA) in situ hybridization (ISH) protocol using dual fluorogenic-labeled oligonucleotide probes composed of a mixture of DNA and locked nucleic acids (LNAs) was developed. This assay was able to differentiate between Aspergillus and Fusarium in formalin-fixed, paraffin-embedded tissue sections. ISH targeting rRNA can be used to identify the species of fungal pathogens in surgical pathology material and may be useful when pathogens are histologically observed but cultures are negative or have not been performed. ISH with dual-labeled LNA probes may be useful for detecting a variety of fungal pathogens in formalin-fixed, paraffin-embedded tissue specimens.

UI	MeSH Term	Description	Entries
D009181	Mycoses	Diseases caused by FUNGI.	Fungus Diseases,Fungal Diseases,Fungal Infections,Fungus Infections,Disease, Fungal,Disease, Fungus,Diseases, Fungal,Diseases, Fungus,Fungal Disease,Fungal Infection,Fungus Disease,Fungus Infection,Infection, Fungal,Infection, Fungus,Infections, Fungal,Infections, Fungus
D009841	Oligonucleotides	Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)	Oligonucleotide
D005557	Formaldehyde	A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)	Formalin,Formol,Methanal,Oxomethane
D005670	Fusarium	A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA.	Fusariums
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001228	Aspergillosis	Infections with fungi of the genus ASPERGILLUS.	Aspergillus Infection,Aspergilloses,Aspergillus Infections,Infection, Aspergillus,Infections, Aspergillus
D001230	Aspergillus	A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
D012331	RNA, Fungal	Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.	Fungal RNA
D012335	RNA, Ribosomal	The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)	Ribosomal RNA,15S RNA,RNA, 15S
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species