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Cytochrome c' from Rhodospirillum rubrum has been investigated in the ferric form with Mössbauer and EPR spectroscopy. In the pH range from 6 to 9.5, three species are observed which belong to two pH-dependent equilibria with pK values near 6 and 8.5. The pK = 6 transition is resolved only with high-field Mössbauer spectroscopy. For the three species we have determined the zero-field splitting parameters and the hyperfine coupling constants. The data were fitted to a spin Hamiltonian which takes into account a weak mixing of excited S = 3/2 states into the sextet ground manifold. The low temperature spectra clearly show that the quadruple coupling constant deltaEQ is positive for ferricytochrome c' and thus in accord with all other high-spin ferric heme proteins.
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
January 1981,
Biochemistry,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
October 1987,
The Journal of biological chemistry,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
May 1976,
Analytical biochemistry,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
April 1979,
Biochemistry,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
June 1973,
The Journal of biological chemistry,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
January 1969,
Archiv fur Mikrobiologie,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
January 1990,
European journal of biochemistry,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
July 1974,
FEBS letters,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
March 1988,
Biochemistry,
M H Emptage, and
R Zimmermann, and
L Que, and
E Münck, and
W D Hamilton, and
W H Orme-Johnson
May 1979,
The Journal of biological chemistry,
