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The formation of paramagnetic non-haem iron dinitrozyl complexes (2,03 complexes) in mouse liver has been detected, when these animals were exposed to a diet with high content of nitrite or nitrite and iron salt. These complexes appeared after 3-5 days and their content was kept at the same level during a month. The 2,03 complexes content in mouse liver was 3 times higher for mice which were exposed to the diet with nitrite and iron salt than for those on the diet only with nitrite. While the liver preparations of experimental mice were treated with NO in vitro the 2,03 complexes content increased in the liver and reached the same level for all experimental mice. It has been supposed that the 2.03 complexes formation was limited in mouse liver in vivo, both by NO production in the liver and by the content of the more labile form of non-haem iron, which was primarily incorporated into the complexes.
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