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B-Z DNA reversible conformation changes effected by high pressure. 1991

A Krzyzaniak, and P Sałański, and J Jurczak, and J Barciszewski
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.

There are numerous data showing that a DNA molecule with alternate pirymidine-purine sequence can adopt a left-handed, double-helical Z-DNA conformation. Such structural changes of DNA occur as a consequence of environmental conditions (e.g. 4 M NaCl) or chemical modification (e.g. methylation or bromination of bases). In this paper, we found for the first time that high pressure (several kilobars) can change the DNA conformation from the B to the Z form. When the pressure is reduced to an atmospheric one, DNA conformation returns back to the B-form. The Z-DNA structure formation was confirmed by circular dichroism (CD) and ultraviolet (UV) measurements. However, we found, that the values of the ratio of absorbance at the wavelengths 295 and 257 nm in the range of 0.3-0.4 is not a fully conclusive proof for the Z-DNA conformation. Although the ratio is typical for Z-DNA form, it is not obvious that the negative band in CD spectrum will be observed. On the other hand, methylated DNA does not undergo B----Z DNA transitions at the high pressure. These conformational changes of DNA molecules could be interpreted as the effect a of different hydration of various DNA forms.

UI MeSH Term Description Entries
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D011312 Pressure A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Pressures
D002942 Circular Dichroism A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Circular Dichroism, Vibrational,Dichroism, Circular,Vibrational Circular Dichroism
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry

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