Comparative protein profiling of serum and plasma using an antibody suspension bead array approach. 2010

Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
Department of Proteomics, School of Biotechnology, KTH - Royal Institute of Technology, Albanova University Center, Stockholm, Sweden. schwenk@kth.se

In the pursuit towards a systematic analysis of human diseases, array-based approaches within antibody proteomics offer high-throughput strategies to discover protein biomarkers in serum and plasma. To investigate the influence of sample preparation on such discovery attempts, we report on a systematic effort to compare serum and plasma protein profiles determined with an antibody suspension bead array. The intensity levels were used to define protein profiles and no significant differences between serum and plasma were observed for 79% of the 174 antibodies (targeting 156 proteins). By excluding 36 antibodies giving rise to differential intensity levels, cluster analysis revealed donor-specific rather than preparation-dependent grouping. With a cohort from a clinically relevant medical condition, the metabolic syndrome, the influence of the sample type on a multiplexed biomarker discovery approach was further investigated. Independent comparisons of protein profiles in serum and plasma revealed an antibody targeting ADAMTSL-4, a protein that would qualify to be studied further in association with the condition. In general, the preparation type had an impact on the results of the applied antibody suspension bead array, and while the technical variability was equal, plasma offered a greater biological variability and allowed to give rise to more discoveries than serum.

UI MeSH Term Description Entries
D001798 Blood Proteins Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins. Blood Protein,Plasma Protein,Plasma Proteins,Serum Protein,Serum Proteins,Protein, Blood,Protein, Plasma,Protein, Serum,Proteins, Blood,Proteins, Plasma,Proteins, Serum
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000071096 ADAMTS Proteins A subfamily of ADAM proteases that are distinguished by the presence of one or more THROMBOSPONDIN type-1 repeats (TSRs). These are three-strand motifs that contain characteristic TRYPTOPHAN, ARGININE, and CYSTEINE residues respectively. In contrast to ADAM proteins, which reside on CELL MEMBRANES, ADAMTS proteases are secreted and function in the EXTRACELLULAR MATRIX. A Disintegrin And Metalloproteinase With Thrombospondin Motifs Protein,ADAMTS Protease,ADAMTS Protein,ADAMTS-Like Protein,ADAMTSL Protein,Adam Metallopeptidases With Thrombospondin Type 1 Motif Protein,A Disintegrin And Metalloproteinase With Thrombospondin Motifs Proteins,ADAMTS Proteases,ADAMTS-Like Proteins,ADAMTSL Proteins,Adam Metallopeptidases With Thrombospondin Type 1 Motif Proteins,ADAMTS Like Protein,ADAMTS Like Proteins,Protease, ADAMTS,Protein, ADAMTS,Protein, ADAMTS-Like,Protein, ADAMTSL
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D015331 Cohort Studies Studies in which subsets of a defined population are identified. These groups may or may not be exposed to factors hypothesized to influence the probability of the occurrence of a particular disease or other outcome. Cohorts are defined populations which, as a whole, are followed in an attempt to determine distinguishing subgroup characteristics. Birth Cohort Studies,Birth Cohort Study,Closed Cohort Studies,Cohort Analysis,Concurrent Studies,Historical Cohort Studies,Incidence Studies,Analysis, Cohort,Cohort Studies, Closed,Cohort Studies, Historical,Studies, Closed Cohort,Studies, Concurrent,Studies, Historical Cohort,Analyses, Cohort,Closed Cohort Study,Cohort Analyses,Cohort Studies, Birth,Cohort Study,Cohort Study, Birth,Cohort Study, Closed,Cohort Study, Historical,Concurrent Study,Historical Cohort Study,Incidence Study,Studies, Birth Cohort,Studies, Cohort,Studies, Incidence,Study, Birth Cohort,Study, Closed Cohort,Study, Cohort,Study, Concurrent,Study, Historical Cohort,Study, Incidence
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D016000 Cluster Analysis A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both. Clustering,Analyses, Cluster,Analysis, Cluster,Cluster Analyses,Clusterings
D054928 Validation Studies as Topic Works on research using processes by which the reliability and relevance of a procedure for a specific purpose are established.
D019699 Thrombospondins A family of related, adhesive glycoproteins which are synthesized, secreted, and incorporated into the extracellular matrix of a variety of cells, including alpha granules of platelets following thrombin activation and endothelial cells. They interact with a number of BLOOD COAGULATION FACTORS and anticoagulant factors. Five distinct forms have been identified, thrombospondin 1, -2, -3, -4, and cartilage oligomeric matrix protein (COMP). They are involved in cell adhesion, platelet aggregation, cell proliferation, angiogenesis, tumor metastasis, VASCULAR SMOOTH MUSCLE growth, and tissue repair. Thrombospondin

Related Publications

Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
January 2013, Methods in molecular biology (Clifton, N.J.),
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
January 2021, Methods in molecular biology (Clifton, N.J.),
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
August 2008, Journal of proteome research,
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
December 2013, Journal of proteomics,
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
November 2006, Journal of inflammation (London, England),
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
January 2011, Methods in molecular biology (Clifton, N.J.),
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
January 2011, Methods in molecular biology (Clifton, N.J.),
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
February 2011, The Analyst,
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
January 2013, Methods in molecular biology (Clifton, N.J.),
Jochen M Schwenk, and Ulrika Igel, and Bernet S Kato, and George Nicholson, and Fredrik Karpe, and Mathias Uhlén, and Peter Nilsson
January 2019, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!