[CpG-oligodeoxynucleotide stimulation improves the success for karyotypic analysis of chronic lymphocytic leukemia cells]. 2009

Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.

OBJECTIVE To explore the effect of CpG-oligodeoxynucleotides (ODN) in chromosome study of chronic lymphocytic leukemia (CLL). METHODS Blood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA, CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization (FISH) were performed. RESULTS The metaphase number>or=20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0%, 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2%, 43.6% and 43.6%, respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%. CONCLUSIONS CpG-ODN DSP30 can effectively raise the detection rate of chromosome aberrations in CLL patients.

UI MeSH Term Description Entries
D007621 Karyotyping Mapping of the KARYOTYPE of a cell. Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009841 Oligonucleotides Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed) Oligonucleotide
D002869 Chromosome Aberrations Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS. Autosome Abnormalities,Cytogenetic Aberrations,Abnormalities, Autosome,Abnormalities, Chromosomal,Abnormalities, Chromosome,Chromosomal Aberrations,Chromosome Abnormalities,Cytogenetic Abnormalities,Aberration, Chromosomal,Aberration, Chromosome,Aberration, Cytogenetic,Aberrations, Chromosomal,Aberrations, Chromosome,Aberrations, Cytogenetic,Abnormalities, Cytogenetic,Abnormality, Autosome,Abnormality, Chromosomal,Abnormality, Chromosome,Abnormality, Cytogenetic,Autosome Abnormality,Chromosomal Aberration,Chromosomal Abnormalities,Chromosomal Abnormality,Chromosome Aberration,Chromosome Abnormality,Cytogenetic Aberration,Cytogenetic Abnormality
D002871 Chromosome Banding Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping. Banding, Chromosome,Bandings, Chromosome,Chromosome Bandings
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly

Related Publications

Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
December 2010, Cancer genetics and cytogenetics,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
December 1986, Cancer genetics and cytogenetics,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
February 2010, Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
June 2015, Oncotarget,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
July 2001, Leukemia & lymphoma,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
December 1991, Cancer genetics and cytogenetics,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
March 2009, Leukemia & lymphoma,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
February 2008, Journal of leukocyte biology,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
April 2020, Zhongguo shi yan xue ye xue za zhi,
Qiong Liu, and Wei Xu, and Hai-rong Qiu, and Rong Wang, and Hui Yu, and Lei Fan, and Kou-rong Miao, and Jian-yong Li
October 1999, Antisense & nucleic acid drug development,
Copied contents to your clipboard!