Multilocus variable-number tandem-repeat analysis and multilocus sequence typing reveal genetic relationships among Clostridium difficile isolates genotyped by restriction endonuclease analysis. 2010

Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
Infectious Diseases Epidemiology Research Unit, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. jwmarsh@pitt.edu

Numbers of Clostridium difficile infections have increased worldwide in the past decade. While infection with C. difficile remains predominantly a health care-associated infection, there may also be an increased incidence of community-associated infections. C. difficile strains of public health significance continue to emerge, and reliable genotyping methods for epidemiological investigations and global surveillance of C. difficile are required. In this study, multilocus sequence typing (MLST) and multilocus variable-number tandem-repeat analysis (MLVA) were performed on a set of 157 spatially and temporally diverse C. difficile isolates that had been previously genotyped by restriction endonuclease analysis (REA) to determine the concordance among these genotyping methods. In addition, sequence analysis of the tcdC genotype was performed to investigate the association of allelic variants with epidemic C. difficile isolates. Overall, the MLST and MLVA data were concordant with REA genotyping data. MLST was less discriminatory than either MLVA or REA, yet this method established C. difficile genetic lineage. MLVA was highly discriminatory and demonstrated relationships among the MLST genetic lineages and REA genotypes that were previously unrecognized. Several tcdC genotypes were specific to epidemic clones, highlighting the possible importance of toxin misregulation in C. difficile disease pathogenesis. This study demonstrates that a combination of MLST and MLVA may prove useful for the investigation and surveillance of emergent C. difficile clones of global public health concern.

UI MeSH Term Description Entries
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D012097 Repressor Proteins Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release. Repressor Molecules,Transcriptional Silencing Factors,Proteins, Repressor,Silencing Factors, Transcriptional
D003015 Clostridium Infections Infections with bacteria of the genus CLOSTRIDIUM and closely related CLOSTRIDIOIDES species. Clostridioides Infections,Clostridioides difficile Infection,Clostridioides perfringens Food Poisoning,Clostridioides perfringens Infections,Clostridioides sordellii Infection,Clostridium difficile Infections,Clostridium sordellii Infections,Clostridium difficile Infection,Clostridium perfringens Food Poisoning,Clostridium perfringens Infections,Clostridium sordellii Infection,Infections, Clostridium,Clostridioides Infection,Clostridioides perfringens Infection,Clostridium Infection,Clostridium perfringens Infection,Infection, Clostridioides difficile,Infection, Clostridioides sordellii,Infection, Clostridium,Infection, Clostridium difficile,Infection, Clostridium sordellii
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000091402 Prohibitins Ubiquitously expressed conserved proteins associated with multiple functions including APOPTOSIS; CELL PROLIFERATION, regulations of various immune and mitochondrial functions, and cellular stress responses. Prohibitin 1 and prohibitin 2 form a ring-shaped complex in the INNER MITOCHONDRIAL MEMBRANE. Prohibitin
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015183 Restriction Mapping Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction

Related Publications

Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
December 2015, Veterinary journal (London, England : 1997),
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
March 2015, Journal of microbiological methods,
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
March 2007, Journal of clinical microbiology,
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
January 2016, Methods in molecular biology (Clifton, N.J.),
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
August 2017, Journal of applied microbiology,
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
August 2011, Journal of medical microbiology,
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
June 2012, Journal of clinical microbiology,
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
May 2020, Tropical animal health and production,
Jane W Marsh, and Mary M O'Leary, and Kathleen A Shutt, and Susan P Sambol, and Stuart Johnson, and Dale N Gerding, and Lee H Harrison
April 2005, Journal of clinical microbiology,
Copied contents to your clipboard!