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In order to develop an immunocytochemical method suitable for the study of the cellular localization and intracellular distribution of polyamines we have prepared and characterized antibodies to polyamines. Artificial immunogens were prepared by coupling putrescine, spermidine and spermine to a carrier protein. Immunogens containing bovine serum albumin as a carrier protein were used to immunize rabbits (polyclonal antibodies) and mice (for the production of Mabs). The specificity of the antibodies was tested in an ELISA system utilizing antigens synthesized from thyroglobulin and one of the polyamines. Polyclonal antibodies to putrescine, spermidine and spermine were obtained. However, these antibodies showed a variable degree of cross-reactivity to the polyamines not used for immunization. Two hybridoma cell lines were developed. The first, MPut88, selectively produces a Mab to putrescine, the second, MSpm/d88 produces a Mab which recognizes spermine and spermidine but does not react with putrescine.
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
September 2003,
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
February 1990,
Biochemical and biophysical research communications,
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
December 2008,
Journal of agricultural and food chemistry,
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
January 1985,
Hypertension (Dallas, Tex. : 1979),
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
March 1989,
Journal of virological methods,
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
August 2005,
Hybridoma (2005),
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
September 1986,
Immunology,
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
June 2001,
Immunology letters,
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
January 2000,
Methods in molecular medicine,
R G Schipper, and
J A Jonis, and
R G Rutten, and
G I Tesser, and
A A Verhofstad
December 1994,
Hybridoma,
