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To understand the molecular basis of gene targeting, we have studied interactions of nucleoprotein filaments comprised of single-stranded DNA and RecA protein with chromatin templates reconstituted from linear duplex DNA and histones. We observed that for the chromatin templates with histone/DNA mass ratios of 0.8 and 1.6, the efficiency of homologous pairing was indistinguishable from that of naked duplex DNA but strand exchange was repressed. In contrast, the chromatin templates with a histone/DNA mass ratio of 9.0 supported neither homologous pairing nor strand exchange. The addition of histone H1, in stoichiometric amounts, to chromatin templates quells homologous pairing. The pairing of chromatin templates with nucleoprotein filaments of RecA protein-single-stranded DNA proceeded without the production of detectable networks of DNA, suggesting that coaggregates are unlikely to be the intermediates in homologous pairing. The application of these observations to strategies for gene targeting and their implications for models of genetic recombination are discussed.
J Ramdas, and
E Mythili, and
K Muniyappa
January 1992,
Journal of molecular biology,
J Ramdas, and
E Mythili, and
K Muniyappa
April 1981,
Proceedings of the National Academy of Sciences of the United States of America,
J Ramdas, and
E Mythili, and
K Muniyappa
July 1980,
Proceedings of the National Academy of Sciences of the United States of America,
J Ramdas, and
E Mythili, and
K Muniyappa
May 1983,
The Journal of biological chemistry,
J Ramdas, and
E Mythili, and
K Muniyappa
January 1991,
Biochimie,
J Ramdas, and
E Mythili, and
K Muniyappa
August 1981,
Nucleic acids research,
J Ramdas, and
E Mythili, and
K Muniyappa
March 2000,
Analytical chemistry,
J Ramdas, and
E Mythili, and
K Muniyappa
March 1991,
The Journal of biological chemistry,
J Ramdas, and
E Mythili, and
K Muniyappa
March 2000,
Genes & development,
J Ramdas, and
E Mythili, and
K Muniyappa
June 1992,
Biochemistry,
