Morphological characterization of baculovirus Autographa californica multiple nucleopolyhedrovirus. 2010

Teemu O Ihalainen, and Johanna P Laakkonen, and Outi Paloheimo, and Seppo Ylä-Herttuala, and Kari J Airenne, and Maija Vihinen-Ranta
NanoScience Center, Department of Biological and Environmental Science, University of Jyväskylä, Jyvaskyla, Finland.

The budded form of baculovirus Autographa californica multiple nucleopolyhedrovirus is used widely in biotechnological applications. In this study, we observed the morphology of baculovirus in nanometer scale by atomic force microscopy. Additionally, the correlation between transduction efficiency and virus stock storage time was evaluated. By atomic force microscopy, asymmetrical baculovirus particles with enlarged head regions were detected. Observed virus stocks contained variable-length particles, 256+/-40nm, along with disintegrated particles and/or cellular components. Long-term storage of stocks led to virus aggregation and decreased cellular entry and transgene expression in mammalian cells. Significantly, baculovirus vectors bearing a 33.5-kb insert induced formation of elongated nucleocapsids, indicating that viral dsDNA genome size influences particle size. Taken together, our results provide the first 3D-characterization of baculovirus morphology and insight into its stability after storage.

UI MeSH Term Description Entries
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014771 Virion The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos. Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D053586 Virus Internalization The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by translocation of the whole virus across the cell membrane, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by fusion of the membrane of infected cells with the membrane of non-infected cells causing SYNCYTIA to be formed. Viral Entry,Viral Internalization,Viral Membrane Fusion,Virus Entry,Virus Membrane Fusion,Entry, Viral,Entry, Virus,Fusion, Viral Membrane,Internalization, Viral,Internalization, Virus,Membrane Fusion, Viral
D017924 Nucleopolyhedroviruses Genera of the family BACULOVIRIDAE, characterized by the formation of crystalline, polyhedral occlusion bodies in the host cell nucleus. Nucleopolyhedrovirus is the common name for viruses from the Alphabaculovirus, Deltabaculovirus, or Gammabaculovirus genera. Deltabaculovirus,Nuclear Polyhedrosis Virus,Nucleopolyhedrovirus,Alphabaculovirus,Gammabaculovirus,Alphabaculoviruses,Deltabaculoviruses,Gammabaculoviruses,Nuclear Polyhedrosis Viruses,Polyhedrosis Virus, Nuclear,Polyhedrosis Viruses, Nuclear,Virus, Nuclear Polyhedrosis,Viruses, Nuclear Polyhedrosis
D018625 Microscopy, Atomic Force A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample. Atomic Force Microscopy,Force Microscopy,Scanning Force Microscopy,Atomic Force Microscopies,Force Microscopies,Force Microscopies, Scanning,Force Microscopy, Scanning,Microscopies, Atomic Force,Microscopies, Force,Microscopies, Scanning Force,Microscopy, Force,Microscopy, Scanning Force,Scanning Force Microscopies
D019251 Nucleocapsid A protein-nucleic acid complex which forms part or all of a virion. It consists of a CAPSID plus enclosed nucleic acid. Depending on the virus, the nucleocapsid may correspond to a naked core or be surrounded by a membranous envelope. Nucleocapsids

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